Ligand field circular dichroism and magnetic circular dichroism studies of component B and substrate binding to the hydroxylase component of methane monooxygenase

Sabine Coates Pulver, Wayne A. Froland, John D. Lipscomb, Edward I. Solomon

Research output: Contribution to journalArticlepeer-review

64 Scopus citations

Abstract

The soluble methane monooxygenase system (MMO), consisting of a hydroxylase (MMOH), a reductase, and component B (MMOB), catalyzes the NADH and O2 dependent hydroxylation of methane and many other hydrocarbons. The binuclear non-heme ferrous active site cluster of the hydroxylase-component B(MMOH-MMOB) complex in the presence of substrate and small molecules has been studied using circular dichroism (CD) and magnetic circular dichroism (MCD) spectroscopies. CD studies reveal that addition of the alternative substrate, trans-1,2-dichloroethylene, or inhibitor, tetrachloroethylene, induces a conformational change in the active site pocket only in the presence of MMOB. Complementary MCD data indicate that this conformational change does not result in a direct change in the ligation of the iron atoms. Comparison of the CD/MCD data with the crystal structure of MMOH allows a tentative correlation between the perturbations observed and the iron atoms affected. The binding of MMOB to MMOH distorts the ligand field environment of one iron, while substrate binding in the presence of MMOB perturbs the other, therefore providing insight into the regulatory role of MMOB and into the participation of the two iron centers in the reaction. Anion binding to the MMOH-MMOB complex was also investigated. No spectral perturbation by small molecules (K(B) < 30 M-1) in reduced MMOH and the MMOH-MMOB complex was observed, suggesting that the hydroxylase active site pocket is less electrophilic than other binuclear non-heme iron proteins, consistent with its role in charge donation to activate dioxygen.

Original languageEnglish (US)
Pages (from-to)387-395
Number of pages9
JournalJournal of the American Chemical Society
Volume119
Issue number2
DOIs
StatePublished - Jan 15 1997

Fingerprint Dive into the research topics of 'Ligand field circular dichroism and magnetic circular dichroism studies of component B and substrate binding to the hydroxylase component of methane monooxygenase'. Together they form a unique fingerprint.

Cite this