Ligand Engineering via Yeast Surface Display and Adherent Cell Panning

Lawrence A. Stern, Patrick S. Lown, Benjamin J. Hackel

Research output: Chapter in Book/Report/Conference proceedingChapter


High-throughput ligand discovery and evolution—via genotype-phenotype linkage strategies—empower molecularly targeted therapy, diagnostics, and fundamental science. Maintaining high-quality target antigen in these selections, particularly for membrane targets, is often a technical challenge. Panning yeast-displayed ligand libraries on intact mammalian cells expressing the molecular target has emerged as an effective strategy. Herein we describe the techniques used to select target-binding ligands via this approach including the use of target-negative cells to deplete non-specific binders and avidity reduction to preferentially select high-affinity ligands.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Number of pages18
StatePublished - Jan 1 2020

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029


  • Avidity
  • Cell panning
  • Depletion
  • Ligand
  • Protein engineering
  • Specificity
  • Yeast surface display

PubMed: MeSH publication types

  • Journal Article
  • Research Support, Non-U.S. Gov't
  • Research Support, N.I.H., Extramural

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  • Cite this

    Stern, L. A., Lown, P. S., & Hackel, B. J. (2020). Ligand Engineering via Yeast Surface Display and Adherent Cell Panning. In Methods in Molecular Biology (pp. 303-320). (Methods in Molecular Biology; Vol. 2070). Humana Press Inc..