Ligand Engineering via Yeast Surface Display and Adherent Cell Panning

Lawrence A. Stern, Patrick S. Lown, Benjamin J. Hackel

Research output: Chapter in Book/Report/Conference proceedingChapter

2 Scopus citations

Abstract

High-throughput ligand discovery and evolution—via genotype-phenotype linkage strategies—empower molecularly targeted therapy, diagnostics, and fundamental science. Maintaining high-quality target antigen in these selections, particularly for membrane targets, is often a technical challenge. Panning yeast-displayed ligand libraries on intact mammalian cells expressing the molecular target has emerged as an effective strategy. Herein we describe the techniques used to select target-binding ligands via this approach including the use of target-negative cells to deplete non-specific binders and avidity reduction to preferentially select high-affinity ligands.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages303-320
Number of pages18
Volume2070
DOIs
StatePublished - 2020

Publication series

NameMethods in molecular biology (Clifton, N.J.)
PublisherHumana Press
ISSN (Print)1064-3745

Bibliographical note

Funding Information:
This chapter describes work funded by the American Cancer Society (130418-RSG-17-110-01-TBG to B.J.H.), the National Institutes of Health (R01 EB023339 to B.J.H.), and the California Tobacco-Related Disease Research Grants Program Office of the University of California (28FT-0072 to L.A.S.).

Publisher Copyright:
© 2020, Springer Science+Business Media, LLC, part of Springer Nature.

Keywords

  • Avidity
  • Cell panning
  • Depletion
  • Ligand
  • Protein engineering
  • Specificity
  • Yeast surface display
  • Humans
  • Peptide Library
  • Animals
  • Protein Engineering
  • Ligands
  • Saccharomyces cerevisiae/chemistry

PubMed: MeSH publication types

  • Research Support, Non-U.S. Gov't
  • Journal Article
  • Research Support, N.I.H., Extramural

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