Ligand Engineering via Yeast Surface Display and Adherent Cell Panning

Lawrence A. Stern, Patrick S. Lown, Benjamin J. Hackel

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

High-throughput ligand discovery and evolution—via genotype-phenotype linkage strategies—empower molecularly targeted therapy, diagnostics, and fundamental science. Maintaining high-quality target antigen in these selections, particularly for membrane targets, is often a technical challenge. Panning yeast-displayed ligand libraries on intact mammalian cells expressing the molecular target has emerged as an effective strategy. Herein we describe the techniques used to select target-binding ligands via this approach including the use of target-negative cells to deplete non-specific binders and avidity reduction to preferentially select high-affinity ligands.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages303-320
Number of pages18
DOIs
StatePublished - Jan 1 2020

Publication series

NameMethods in Molecular Biology
Volume2070
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Fingerprint

Yeasts
Ligands
Genotype
Phenotype
Antigens
Membranes
Therapeutics

Keywords

  • Avidity
  • Cell panning
  • Depletion
  • Ligand
  • Protein engineering
  • Specificity
  • Yeast surface display

PubMed: MeSH publication types

  • Journal Article

Cite this

Stern, L. A., Lown, P. S., & Hackel, B. J. (2020). Ligand Engineering via Yeast Surface Display and Adherent Cell Panning. In Methods in Molecular Biology (pp. 303-320). (Methods in Molecular Biology; Vol. 2070). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-9853-1_17

Ligand Engineering via Yeast Surface Display and Adherent Cell Panning. / Stern, Lawrence A.; Lown, Patrick S.; Hackel, Benjamin J.

Methods in Molecular Biology. Humana Press Inc., 2020. p. 303-320 (Methods in Molecular Biology; Vol. 2070).

Research output: Chapter in Book/Report/Conference proceedingChapter

Stern, LA, Lown, PS & Hackel, BJ 2020, Ligand Engineering via Yeast Surface Display and Adherent Cell Panning. in Methods in Molecular Biology. Methods in Molecular Biology, vol. 2070, Humana Press Inc., pp. 303-320. https://doi.org/10.1007/978-1-4939-9853-1_17
Stern LA, Lown PS, Hackel BJ. Ligand Engineering via Yeast Surface Display and Adherent Cell Panning. In Methods in Molecular Biology. Humana Press Inc. 2020. p. 303-320. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4939-9853-1_17
Stern, Lawrence A. ; Lown, Patrick S. ; Hackel, Benjamin J. / Ligand Engineering via Yeast Surface Display and Adherent Cell Panning. Methods in Molecular Biology. Humana Press Inc., 2020. pp. 303-320 (Methods in Molecular Biology).
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