A unique method that applied a multilayer-immobilization strategy was developed to prepare nanofibrous enzymes for biosynthesis. LiCl co-electrospun with polyurethane nanofibers enabled strong physical adsorption of bovine serum albumin (BSA), forming the first layer of protein on the nanofibers; lipase AK was subsequently crosslinked to BSA as an outer layer of enzyme. The content of LiCl in nanofibers was found to be a sensitive factor affecting the activity and stability of the immobilized lipase. For biodiesel synthesis from soybean oil and methanol in isooctane, the reaction rate catalyzed by nanofibrious lipase carrying 5. wt% LiCl was 6.6-fold higher than fibers without LiCl, with a conversion of 91% was achieved within 2. h. LiCl also induced much improved enzyme stability. The nanofibrous lipase with 5% LiCl could be repeatedly used for 42 cycles without apparent activity loss, while the immobilized lipase without LiCl lost over 90% activity within 13 reuse cycles.
Bibliographical noteFunding Information:
This work was financially supported by 863 Project ( 2008AA10Z302 ), National Natural Science Foundation of China ( 20706054 , 20728607 , 20976180 ), and 973 project ( 2009CB724705 ).
- Lipase AK
- Polyurethane nanofibers