LiCl-induced improvement of multilayer nanofibrous lipase for biodiesel synthesis

Chun Xia Liu, Song Ping Zhang, Zhi Guo Su, Ping Wang

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

A unique method that applied a multilayer-immobilization strategy was developed to prepare nanofibrous enzymes for biosynthesis. LiCl co-electrospun with polyurethane nanofibers enabled strong physical adsorption of bovine serum albumin (BSA), forming the first layer of protein on the nanofibers; lipase AK was subsequently crosslinked to BSA as an outer layer of enzyme. The content of LiCl in nanofibers was found to be a sensitive factor affecting the activity and stability of the immobilized lipase. For biodiesel synthesis from soybean oil and methanol in isooctane, the reaction rate catalyzed by nanofibrious lipase carrying 5. wt% LiCl was 6.6-fold higher than fibers without LiCl, with a conversion of 91% was achieved within 2. h. LiCl also induced much improved enzyme stability. The nanofibrous lipase with 5% LiCl could be repeatedly used for 42 cycles without apparent activity loss, while the immobilized lipase without LiCl lost over 90% activity within 13 reuse cycles.

Original languageEnglish (US)
Pages (from-to)266-272
Number of pages7
JournalBioresource Technology
Volume103
Issue number1
DOIs
StatePublished - Jan 2012

Bibliographical note

Funding Information:
This work was financially supported by 863 Project ( 2008AA10Z302 ), National Natural Science Foundation of China ( 20706054 , 20728607 , 20976180 ), and 973 project ( 2009CB724705 ).

Keywords

  • Biodiesel
  • LiCl
  • Lipase AK
  • Polyurethane nanofibers
  • Stability

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