Trypanosoma brucei spp. cause African human and animal trypanosomiasis, a burden on health and economy in Africa. These hemoflagellates are distinguished by a kinetoplast nucleoid containing mitochondrial DNAs of two kinds: maxicircles encoding ribosomal RNAs (rRNAs) and proteins and minicircles bearing guide RNAs (gRNAs) for mRNA editing. All RNAs are produced by a phage-type RNA polymerase as 3′ extended precursors, which undergo exonucleolytic trimming. Most pre-mRNAs proceed through 3′ adenylation, uridine insertion/deletion editing, and 3′ A/U-tailing. The rRNAs and gRNAs are 3′ uridylated. Historically, RNA editing has attracted major research effort, and recently essential pre- and postediting processing events have been discovered. Here, we classify the key players that transform primary transcripts into mature molecules and regulate their function and turnover.
Bibliographical noteFunding Information:
Aphasizheva lab is supported by NIH grant AI113157 . Aphasizhev lab is supported by NIH grants AI091914 and AI101057 . Cestari lab is supported by NSERC grants RGPIN-2019-04658 and DGECR-2019-00081 . Cruz-Reyes lab is supported by NSF grant 1616845 and TAMU X-grant and T3 award. Göringer lab is supported by German Research Council ( SPP1857 ). Lukeš, Yurchenko and Zíková labs are supported by the Czech Ministry of Education ( OPVVV16_019/0000759 ), ERC CZ LL1601 , and Czech Grant Agency grants 18-17529S and 18-15962S . Read lab is supported by NIH grants AI061580 , GM129041 , and AI139448 . Hajduk lab is supported by NIH grant AI125487 . Schnaufer lab is supported by MRC Senior Non-Clinical Fellowship MR/L019701/1 . Schneider lab is supported by the NCCR grant "RNA & Disease" and by the grant 175563 from the Swiss National Science Foundation . Stuart lab is supported by NIH grant 5R01AI014102-43 . Zimmer lab is supported by NIH grant AI135885 .
- RNA decay
- RNA editing
PubMed: MeSH publication types
- Journal Article
- Research Support, N.I.H., Extramural
- Research Support, Non-U.S. Gov't