Leukocyte migration inhibition in chickens inoculated with Salmonella typhimurium.

K. V. Nagaraja, J. A. Newman, B. S. Pomeroy

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Leukocyte migration-inhibition tests were conducted in chickens infected with Salmonella typhimurium. A relatively new and simple method that involves the migration of leukocytes under agarose gel was used. Migration distance was projected onto a white background at 40X magnification, using a microprojector. Quantitation of migration was done by measuring the linear distance the cells had moved from the margin of the agarose wells. The details of the technique and the importance of it in studies of cell-mediated immune response are discussed. Chickens inoculated with S typhimurium had a significant leukocyte migration inhibition.

Original languageEnglish (US)
Pages (from-to)916-918
Number of pages3
JournalAmerican journal of veterinary research
Volume43
Issue number5
StatePublished - May 1 1982

Fingerprint

Salmonella typhimurium
Salmonella Typhimurium
Sepharose
Chickens
leukocytes
Leukocytes
Leukocyte Cell Migration Assays
chickens
agarose
Gels
cell-mediated immunity
gels
methodology
testing
cells

Cite this

Leukocyte migration inhibition in chickens inoculated with Salmonella typhimurium. / Nagaraja, K. V.; Newman, J. A.; Pomeroy, B. S.

In: American journal of veterinary research, Vol. 43, No. 5, 01.05.1982, p. 916-918.

Research output: Contribution to journalArticle

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