Leukocyte chemotaxis under agarose: Manipulations of serum and plasma before incorporation into agarose can influence cell movement

Mark V Dahl, William E. Lindroos

Research output: Contribution to journalArticle

11 Scopus citations

Abstract

In the migration under agarose assay for measurement of polymorphonuclear leukocyte chemotaxis and random migration, the agarose gel ordinarily contains 10% human serum or plasma. Influences of serum and plasma on cell movement using this assay were defined. Utilizing plasma as protein source allowed more random migration and chemotaxis than when serum was used. Heat inactivation of both serum and plasma decreased random migration and chemotaxis. If heating at 56°C was prolonged beyond 15-30 min, leukocyte movement was augmented. Excessive concentrations of platelets or platelet products, or heparin in agarose, also augmented migration, but storage of plasma or sera at various temperatures for 24 h did not appreciably affect migration. Large amounts of hemoglobin in agarose decreased chemotaxis, but the effect of trace amounts of hemolysis was negligible. Handling plasma or sera in standard ways prior to use in the under agarose assay may make this assay more reproducible and sensitive.

Original languageEnglish (US)
Pages (from-to)301-310
Number of pages10
JournalJournal of Immunological Methods
Volume29
Issue number4
DOIs
StatePublished - Sep 24 1979

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