Leukocidal activity of staphylococci isolated from human periodontal lesions

Masayasu Iwase, Jørgen Slots, Peter Berthold, Norton S. Taichman

Research output: Contribution to journalArticle

12 Scopus citations

Abstract

Staphylococci isolated from subgingival samples of patients with advanced periodontitis were tested for leukocidal activity. Intact organisms, bacterial sonicates or bacterial culture supernatants were incubated with human neutrophils that had been prelabeled with 51chromium. The majority of Staphylococcus aureus periodontal isolates provoked dose–dependent extracellular release of the radiolabel. by contrast, other strains of staphylococci had marginal or no demonstrable lukocidal activity. Leukocidal activity was heat‐sensitive and was neutralized by horse anti‐leukocidin antibodies. The results indicate that 5. aureus colonizing human periodontal lesions elaborate a leukocidin that may conceivably destroy neutrophils that emigrate into the gingival region. This could adversely affect the overall status of this antimicrobial defense system in the gingival area and contribute to the pathogenicity of S. aureus as well as other potential periodontopathic organisms.

Original languageEnglish (US)
Pages (from-to)233-236
Number of pages4
JournalOral Microbiology and Immunology
Volume5
Issue number4
DOIs
StatePublished - Aug 1990

Keywords

  • Staphylococcus aureus
  • cytotoxicity
  • leukocidin
  • neutrophil
  • periodontal Staphylococcus

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