Lesion Profiling and Subcellular Prion Localization of Cervid Chronic Wasting Disease in Domestic Cats

Davis Seelig, A. V. Nalls, M. Flasik, V. Frank, S. Eaton, C. K. Mathiason, E. A. Hoover

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Chronic wasting disease (CWD) is an efficiently transmitted, fatal, and progressive prion disease of cervids with an as yet to be fully clarified host range. While outbred domestic cats (Felis catus) have recently been shown to be susceptible to experimental CWD infection, the neuropathologic features of the infection are lacking. Such information is vital to provide diagnostic power in the event of natural interspecies transmission and insights into host and strain interactions in interspecies prion infection. Using light microscopy and immunohistochemistry, we detail the topographic pattern of neural spongiosis (the “lesion profile”) and the distribution of misfolded prion protein in the primary and secondary passage of feline CWD (FelCWD). We also evaluated cellular and subcellular associations between misfolded prion protein (PrPD) and central nervous system neurons and glial cell populations. From these studies, we (1) describe the novel neuropathologic profile of FelCWD, which is distinct from either cervid CWD or feline spongiform encephalopathy (FSE), and (2) provide evidence of serial passage-associated interspecies prion adaptation. In addition, we demonstrate through confocal analysis the successful co-localization of PrPD with neurons, astrocytes, microglia, lysosomes, and synaptophysin, which, in part, implicates each of these in the neuropathology of FelCWD. In conclusion, this work illustrates the simultaneous role of both host and strain in the development of a unique FelCWD neuropathologic profile and that such a profile can be used to discriminate between FelCWD and FSE.

Original languageEnglish (US)
Pages (from-to)107-119
Number of pages13
JournalVeterinary pathology
Volume52
Issue number1
DOIs
StatePublished - Jan 29 2015

Fingerprint

Chronic Wasting Disease
chronic wasting disease
Prions
prions
Felidae
lesions (animal)
Cats
cats
prion diseases
Brain Diseases
neuroglia
Infection
Serial Passage
neurons
Neurons
infection
Synaptophysin
Prion Diseases
neuropathology
Host Specificity

Keywords

  • adaptation
  • cat
  • chronic wasting disease
  • feline spongiform encephalopathy
  • immunohistochemistry
  • interspecies
  • prion
  • species barrier
  • transmissible spongiform encephalopathy

Cite this

Lesion Profiling and Subcellular Prion Localization of Cervid Chronic Wasting Disease in Domestic Cats. / Seelig, Davis; Nalls, A. V.; Flasik, M.; Frank, V.; Eaton, S.; Mathiason, C. K.; Hoover, E. A.

In: Veterinary pathology, Vol. 52, No. 1, 29.01.2015, p. 107-119.

Research output: Contribution to journalArticle

Seelig, Davis ; Nalls, A. V. ; Flasik, M. ; Frank, V. ; Eaton, S. ; Mathiason, C. K. ; Hoover, E. A. / Lesion Profiling and Subcellular Prion Localization of Cervid Chronic Wasting Disease in Domestic Cats. In: Veterinary pathology. 2015 ; Vol. 52, No. 1. pp. 107-119.
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