Leptin-induced changes in body composition in high fat-fed mice

Ruth B.S. Harris, Tiffany D. Mitchell, Sadie Hebert

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Female C57BL/6J mice were adapted to 10% or 45% kcal fat diets for 8 weeks. Continuous intraperitoneal infusion of 10 μg of leptin/day from a miniosmotic pump transiently inhibited food intake in low fat-fed but not high fat-fed mice. In contrast, both low and high fat-fed leptin-infused mice were less fat than their phosphate-buffered saline (PBS) controls after 13 days. Leptin infusion inhibited insulin release but did not change glucose clearance in low fat-fed mice during a glucose tolerance test. A single intraperitoneal injection of 30 μg of leptin inhibited 24-hr energy intake and inhibited weight gain in both low and high fat-fed mice. Insulin responsiveness was improved in high fat-fed mice during an insulin sensitivity test due to an exaggerated elevation of circulating insulin concentrations. Thus, leptin infusion reduced adiposity independently of energy intake in high fat-fed mice and improved insulin sensitivity in low fat-fed mice, whereas leptin injections, which produced much greater, but transient, increases in serum leptin concentration, inhibited energy intake in both low and high fat-fed mice.

Original languageEnglish (US)
Pages (from-to)24-32
Number of pages9
JournalExperimental Biology and Medicine
Volume228
Issue number1
StatePublished - Jan 1 2003

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Leptin
Body Composition
Fats
Chemical analysis
Insulin
Energy Intake
Insulin Resistance
Parenteral Infusions
Glucose
Adiposity
Glucose Tolerance Test
Nutrition
Intraperitoneal Injections
Inbred C57BL Mouse
Weight Gain
Eating
Phosphates
Pumps
Diet
Injections

Keywords

  • Body composition
  • IRS protein
  • Leptin infusion
  • Leptin injection
  • Leptin resistance

Cite this

Leptin-induced changes in body composition in high fat-fed mice. / Harris, Ruth B.S.; Mitchell, Tiffany D.; Hebert, Sadie.

In: Experimental Biology and Medicine, Vol. 228, No. 1, 01.01.2003, p. 24-32.

Research output: Contribution to journalArticle

Harris, Ruth B.S. ; Mitchell, Tiffany D. ; Hebert, Sadie. / Leptin-induced changes in body composition in high fat-fed mice. In: Experimental Biology and Medicine. 2003 ; Vol. 228, No. 1. pp. 24-32.
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