LED arrays as cost effective and efficient light sources for widefield microscopy

Dinu F. Albeanu, Edward Soucy, Tomokazu F. Sato, Markus Meister, Venkatesh N. Murthy

Research output: Contribution to journalArticlepeer-review

45 Scopus citations

Abstract

New developments in fluorophores as well as in detection methods have fueled the rapid growth of optical imaging in the life sciences. Commercial widefield microscopes generally use arc lamps, excitation/ emission filters and shutters for fluorescence imaging. These components can be expensive, difficult to maintain and preclude stable illumination. Here, we describe methods to construct inexpensive and easy-to-use light sources for optical microscopy using light-emitting diodes (LEDs). We also provide examples of its applicability to biological fluorescence imaging.

Original languageEnglish (US)
Article numbere2146
JournalPloS one
Volume3
Issue number5
DOIs
StatePublished - May 14 2008

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