Leaf spray mass spectrometry

A rapid ambient ionization technique to directly assess metabolites from plant tissues

Dana M. Freund, Katherine A. Sammons, Nokwanda P. Makunga, Jerry D. Cohen, Adrian D. Hegeman

Research output: Contribution to journalArticle

Abstract

Plants produce thousands of small molecules that are diverse in their chemical properties. Mass spectrometry (MS) is a powerful technique for analyzing plant metabolites because it provides molecular weights with high sensitivity and specificity. Leaf spray MS is an ambient ionization technique where plant tissue is used for direct chemical analysis via electrospray, eliminating chromatography from the process. This approach to sampling metabolites allows for a wide range of chemical classes to be detected simultaneously from intact plant tissues, minimizing the amount of sample preparation needed. When used with a high-resolution, accurate mass MS, leaf spray MS facilitates the rapid detection of metabolites of interest. It is also possible to collect tandem mass fragmentation data with this technique to facilitate a compound identification. The combination of accurate mass measurements and fragmentation is beneficial in confirming compound identities. The leaf spray MS technique requires only minor modifications to a nanospray ionization source and is a useful tool to further expand the capabilities of a mass spectrometer. Here, fresh leaf tissue from Sceletium tortuosum (Aizoaceae), a traditional medicinal plant from South Africa, is analyzed; numerous mesembrine alkaloids are detected with leaf spray MS.

Original languageEnglish (US)
Article numbere57949
JournalJournal of Visualized Experiments
Volume2018
Issue number136
DOIs
StatePublished - Jun 21 2018

Fingerprint

Metabolites
Ionization
Mass spectrometry
Mass Spectrometry
Tissue
Aizoaceae
Alkaloids
Mass spectrometers
Ion sources
Medicinal Plants
South Africa
Chromatography
Chemical properties
Molecular Weight
Molecular weight
Sampling
Sensitivity and Specificity
Molecules
Chemical analysis

Keywords

  • Ambient ionization
  • Biochemistry
  • Electrospray ionization
  • Issue 136
  • Leaf spray MS
  • Mass spectrometry
  • Mesembrine alkaloids
  • Natural products
  • Plant metabolites
  • Sceletium tortuosum
  • Small molecules

PubMed: MeSH publication types

  • Journal Article
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Video-Audio Media

Cite this

Leaf spray mass spectrometry : A rapid ambient ionization technique to directly assess metabolites from plant tissues. / Freund, Dana M.; Sammons, Katherine A.; Makunga, Nokwanda P.; Cohen, Jerry D.; Hegeman, Adrian D.

In: Journal of Visualized Experiments, Vol. 2018, No. 136, e57949, 21.06.2018.

Research output: Contribution to journalArticle

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