Abstract
Apo E plays an important role in chylomicron and VLDL remnant processing, uptake or conversion to LDL. The type of lipoprotein that isolates in the LDL density of E2/2 subjects was investigated and the effect of the apo E isoforms on LDL mass was determined in all genotypes in a large group of Type 1 diabetics. Analysis of the LDL composition of E2/2 homozygotes (n = 6) compared to subjects with the common E3/3 isoform (n = 6) demonstrated an enrichment in apo E, unesterified cholesterol, phospholipid and triglyceride relative to apo B in E2/2 subjects, more typical of a dense IDL remnant than of LDL. Although diabetics were studied, these findings are considered to reflect those of the general population. Comparison of the lipoprotein distribution of homozygous and heterozygous subjects revealed that, as genotype changed from E4/4 (n = 22) to E3/4 (n = 262), E3/3 (n = 710) = E2/4 (n = 30), E2/3 (n = 151), E2/2 (n = 6), LDL cholesterol decreased significantly in a stepwise manner. The decrease was not in a specific subgroup of LDL. In conclusion, for E2/2 subjects, lipoproteins isolated in the LDL density range appear to be composed mainly of dense IDL remnants and some Lp(a). The apo E isoform also has a significant effect on LDL concentration in both homozygotes and heterozygotes.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 138-147 |
| Number of pages | 10 |
| Journal | Atherosclerosis |
| Volume | 192 |
| Issue number | 1 |
| DOIs | |
| State | Published - May 1 2007 |
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Keywords
- Apo E genotype
- E2/2
- LDL
- LDL composition
- VLDL remnants
Cite this
LDL composition in E2/2 subjects and LDL distribution by Apo E genotype in type 1 diabetes. / Murdoch, Susan J.; Boright, Andrew P.; Paterson, Andrew D.; Zinman, Bernard; Steffes, Michael W; Cleary, Patricia; Edwards, Karen; Marcovina, Santica S.; Purnell, Jonathan Q.; Brunzell, John D.
In: Atherosclerosis, Vol. 192, No. 1, 01.05.2007, p. 138-147.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - LDL composition in E2/2 subjects and LDL distribution by Apo E genotype in type 1 diabetes
AU - Murdoch, Susan J.
AU - Boright, Andrew P.
AU - Paterson, Andrew D.
AU - Zinman, Bernard
AU - Steffes, Michael W
AU - Cleary, Patricia
AU - Edwards, Karen
AU - Marcovina, Santica S.
AU - Purnell, Jonathan Q.
AU - Brunzell, John D.
PY - 2007/5/1
Y1 - 2007/5/1
N2 - Apo E plays an important role in chylomicron and VLDL remnant processing, uptake or conversion to LDL. The type of lipoprotein that isolates in the LDL density of E2/2 subjects was investigated and the effect of the apo E isoforms on LDL mass was determined in all genotypes in a large group of Type 1 diabetics. Analysis of the LDL composition of E2/2 homozygotes (n = 6) compared to subjects with the common E3/3 isoform (n = 6) demonstrated an enrichment in apo E, unesterified cholesterol, phospholipid and triglyceride relative to apo B in E2/2 subjects, more typical of a dense IDL remnant than of LDL. Although diabetics were studied, these findings are considered to reflect those of the general population. Comparison of the lipoprotein distribution of homozygous and heterozygous subjects revealed that, as genotype changed from E4/4 (n = 22) to E3/4 (n = 262), E3/3 (n = 710) = E2/4 (n = 30), E2/3 (n = 151), E2/2 (n = 6), LDL cholesterol decreased significantly in a stepwise manner. The decrease was not in a specific subgroup of LDL. In conclusion, for E2/2 subjects, lipoproteins isolated in the LDL density range appear to be composed mainly of dense IDL remnants and some Lp(a). The apo E isoform also has a significant effect on LDL concentration in both homozygotes and heterozygotes.
AB - Apo E plays an important role in chylomicron and VLDL remnant processing, uptake or conversion to LDL. The type of lipoprotein that isolates in the LDL density of E2/2 subjects was investigated and the effect of the apo E isoforms on LDL mass was determined in all genotypes in a large group of Type 1 diabetics. Analysis of the LDL composition of E2/2 homozygotes (n = 6) compared to subjects with the common E3/3 isoform (n = 6) demonstrated an enrichment in apo E, unesterified cholesterol, phospholipid and triglyceride relative to apo B in E2/2 subjects, more typical of a dense IDL remnant than of LDL. Although diabetics were studied, these findings are considered to reflect those of the general population. Comparison of the lipoprotein distribution of homozygous and heterozygous subjects revealed that, as genotype changed from E4/4 (n = 22) to E3/4 (n = 262), E3/3 (n = 710) = E2/4 (n = 30), E2/3 (n = 151), E2/2 (n = 6), LDL cholesterol decreased significantly in a stepwise manner. The decrease was not in a specific subgroup of LDL. In conclusion, for E2/2 subjects, lipoproteins isolated in the LDL density range appear to be composed mainly of dense IDL remnants and some Lp(a). The apo E isoform also has a significant effect on LDL concentration in both homozygotes and heterozygotes.
KW - Apo E genotype
KW - E2/2
KW - LDL
KW - LDL composition
KW - VLDL remnants
UR - http://www.scopus.com/inward/record.url?scp=34247103197&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=34247103197&partnerID=8YFLogxK
U2 - 10.1016/j.atherosclerosis.2006.04.023
DO - 10.1016/j.atherosclerosis.2006.04.023
M3 - Article
C2 - 16740268
AN - SCOPUS:34247103197
VL - 192
SP - 138
EP - 147
JO - Atherosclerosis
JF - Atherosclerosis
SN - 0021-9150
IS - 1
ER -