LCC15-MB cells are MDA-MB-435: A review of misidentified breast and prostate cell lines

Erik W. Thompson, Mark Waltham, Susan J. Ramus, Anne Marie Hutchins, Jane E. Armes, Ian G. Campbell, Elizabeth D. Williams, Phillip R. Thompson, James M. Rae, Michael D. Johnson, Robert Clarke

Research output: Contribution to journalArticlepeer-review

14 Scopus citations


Current stocks of the LCC15-MB cell line, which we originally isolated from a human breast-bone metastasis, were found to be genetically matched to the MDA-MB-435 cell line from the Lombardi Cancer Center (MDA-MB-435-LCC) using comparative genomic hybridisation, DNA microsatellite analysis and chromosomal number. LCC15-MB stocks used for our previously published studies as well as the earliest available LCC15-MB cells also showed identity to MDA-MB-435-LCC cells. The original karyotype reported for LCC15-MB cells was considerably different to that of MDA-MB-435 cells, indicating that the original LCC15-MB cells were lost to contamination by MDA-MB-435-LCC cells. Chromosome number is the simplest test to distinguish original LCC 15-MB cells (n ∼ 75) from MDA-MB-435 (n ∼ 52). Collectively, our results prove that LCC15-MB cells currently available are MDA-MB-435 cells and we suggest their re-designation as MDA-MB-435-LCC15 cells. We also review the known misclassification of breast and prostate cancer cell lines to date and have initiated a register maintained at

Original languageEnglish (US)
Pages (from-to)535-541
Number of pages7
JournalClinical and Experimental Metastasis
Issue number6
StatePublished - Oct 2004
Externally publishedYes

Bibliographical note

Funding Information:
This work was primarily supported by the Victorian Breast Cancer Research Consortium, and in part by Breast Cancer Research Foundation grant N003173, US-DOD IDEA grant (BC 021320) and NIH Par 99-128: Insight Awards to Stamp Out Breast Cancer (1 R21 CA87244-01). We thank the Microscopy and Imaging, Tissue Culture, Biostatistics, Research Computing, and Macromolecular Shared Resources of the Lombardi Cancer Center, which are partially supported by PHS grant NIH 1P30-CA-51008 (Cancer Center Support Grant) to Lombardi Cancer Center.


  • Breast cancer
  • Cell lines
  • Chromosomal number
  • LCC15-MB
  • MDA-MB-435
  • Microsatellite analysis
  • Misidentification


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