Layer plate CAS assay for the quantitation of siderophore production and determination of exudation patterns for fungi

Megan Y. Andrews; Cara M. Santelli; Owen W. Duckworth

doi:10.1016/j.mimet.2015.12.012

Layer plate CAS assay for the quantitation of siderophore production and determination of exudation patterns for fungi

Megan Y. Andrews
, Cara M. Santelli
, Owen W. Duckworth

Earth and Environmental Sciences-Twin Cities

Research output: Contribution to journal › Article › peer-review

29 Scopus citations

Abstract

The chrome azurol S (CAS) assay measures the chelating activity of siderophores, but its application (especially to fungi) is limited by toxicity issues. In this note, we describe a modified version of the CAS assay that is suitable for quantifying siderophore exudation for microorganisms, including fungi.

Original language	English (US)
Pages (from-to)	41-43
Number of pages	3
Journal	Journal of Microbiological Methods
Volume	121
DOIs	https://doi.org/10.1016/j.mimet.2015.12.012
State	Published - Feb 1 2016

Bibliographical note

Publisher Copyright:
© 2015 Elsevier B.V.

Keywords

CAS assay
Fungi
Quantitative assay
Siderophores

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10.1016/j.mimet.2015.12.012

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title = "Layer plate CAS assay for the quantitation of siderophore production and determination of exudation patterns for fungi",

abstract = "The chrome azurol S (CAS) assay measures the chelating activity of siderophores, but its application (especially to fungi) is limited by toxicity issues. In this note, we describe a modified version of the CAS assay that is suitable for quantifying siderophore exudation for microorganisms, including fungi.",

keywords = "CAS assay, Fungi, Quantitative assay, Siderophores",

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doi = "10.1016/j.mimet.2015.12.012",

language = "English (US)",

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AU - Andrews, Megan Y.

AU - Santelli, Cara M.

AU - Duckworth, Owen W.

PY - 2016/2/1

Y1 - 2016/2/1

N2 - The chrome azurol S (CAS) assay measures the chelating activity of siderophores, but its application (especially to fungi) is limited by toxicity issues. In this note, we describe a modified version of the CAS assay that is suitable for quantifying siderophore exudation for microorganisms, including fungi.

AB - The chrome azurol S (CAS) assay measures the chelating activity of siderophores, but its application (especially to fungi) is limited by toxicity issues. In this note, we describe a modified version of the CAS assay that is suitable for quantifying siderophore exudation for microorganisms, including fungi.

KW - CAS assay

KW - Fungi

KW - Quantitative assay

KW - Siderophores

UR - https://www.scopus.com/pages/publications/84953386448

UR - https://www.scopus.com/pages/publications/84953386448#tab=citedBy

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DO - 10.1016/j.mimet.2015.12.012

M3 - Article

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AN - SCOPUS:84953386448

SN - 0167-7012

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SP - 41

EP - 43

JO - Journal of Microbiological Methods

JF - Journal of Microbiological Methods

ER -

Layer plate CAS assay for the quantitation of siderophore production and determination of exudation patterns for fungi

Abstract

Bibliographical note

Keywords

Publisher link

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