LASER-INDUCED GRAPHENE HEATER FOR DNA AMPLIFICATION AND CRISPR-CAS9 BIOSENSING

This paper presents an innovative approach by integrating laser-induced graphene (LIG) as a low-power heater and a CRISPR-Cas9 sensor for efficient DNA amplification and detection. LIG, engineered using a laser-induced carbonization technique on polyimide tape, serves as a rapid heater for isothermal DNA amplification, achieving results within 30 minutes with minimal power consumption. Simultaneously, a CRISPR-Cas9 sensor detects DNA concentrations as low as 16 pM, offering cost-effective and sensitive detection. The combination of LIG heaters and CRISPR-Cas9 sensors streamlines DNA amplification and detection processes, offering substantial advantages over traditional methods in terms of power efficiency, cost effectiveness, and application simplicity. We validated our approach using lateral flow assay technology. Further, a pyrolytic glassy carbon electrode-based electrochemical sensor demonstrates specificity in DNA detection, with a detection limit of 16 pM. This integrated approach holds promise for multiple applications, particularly in nucleic acid diagnostics, showcasing a resource-efficient strategy for DNA amplification and detection in molecular biology research.