Langerhans cells are not required for epidermal Vγ3 T cell homeostasis and function

Sylvie Taveirne, Veerle de Colvenaer, Tina van den Broeck, Els van Ammel, Clare L. Bennett, Tom Taghon, Bart Vandekerckhove, Jean Plum, Björn E. Clausen, Daniel H. Kaplan, Georges Leclercq

Research output: Contribution to journalArticlepeer-review

7 Scopus citations


This study tested the hypothesis that Vγ3 TCR-bearing T cells are influenced by LCs. Vγ3 T cells and LCs are located in the epidermis of mice. Vγ3 T cells represent the main T cell population in the skin epithelium and play a crucial role in maintaining the skin integrity, whereas LCs are professional APCs. Although Vγ3 T cells and LCs form an interdigitating network in the epidermis, not much is known about their reciprocal influence and/or interdependence. We used two different LC-deficient mouse models, in which LCs are constitutively or inducibly depleted, to investigate the role of LCs in maturation, homeostasis, and function of Vγ3 T cells. We show that Vγ3 T cell numbers are unaltered by LC deficiency, and Vγ3 T cells isolated from LC-deficient mice are phenotypically and upon in vitro stimulation, functionally indistinguishable from Vγ3 T cells isolated from WT mice based on their cytotoxic potential and cytokine production. Additionally, in vivo skinwounding experiments show no major difference in response of Vγ3 T cells to wounding in the absence or presence of LCs. These observations indicate that Vγ3 T cells develop and function independently of LCs.

Original languageEnglish (US)
Pages (from-to)61-68
Number of pages8
JournalJournal of Leukocyte Biology
Issue number1
StatePublished - Jul 2011
Externally publishedYes


  • LC deficiency
  • Langerin DTA mice
  • Langerin DTR mice
  • Skin


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