Abstract
Non-autoimmune prone CBA mice were compared with autoimmune prone NZB, NZW, and (NZB × NZW)F1 mice for the ability of their splenic cells to produce anti-ssDNA-forming cells spontaneously in vitro, measured in the plaque forming cell assay. The number of antibody forming cells was measured and the relative avidity of antibody produced determined using a plaque inhibition assay. Splenic lymphocytes from young animals of a non-autoimmune strain (CBAIJ) were shown to be capable of generating anti-ssDNA IgM antibody-forming cells in culture which displayed a higher avidity for antigen than that from autoimmune-prone or frankly autoimmune mice. Since an increased switching from IgM to IgG autoantibody production and defects in Fc-mediated signalling by IgG antibody have been identified in autoimmunity, we suggest that the metabolic block, normally in force in non-autoimmune-prone animals, accounts for this elevated avidity of IgM autoantibody.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 381-389 |
| Number of pages | 9 |
| Journal | International Immunology |
| Volume | 2 |
| Issue number | 5 |
| DOIs | |
| State | Published - May 1990 |
Bibliographical note
Funding Information:This work was supported by the Arthritis Society of Canada, and is currently funded by the Medical Research Council of Canada (Cell Physiology) and by the Raymond C Raymond Foundation, which provided a studentship to one of us (A.P.). We wish to acknowledge the expert technical assistance of Luan Chau, Nicki Panoskaltsis, Rahbar Rahimpour, and Colin Anderson.
Keywords
- End product feedback
- Immunoregulation
- Regulation by Fc signals
- Regulation of antibody responses
- Self-non-self discrimination