TY - JOUR
T1 - Lack of high avidity IgM anti-ssDNA antibodies in cells from autoimmune-prone and autoimmune mice
AU - Panoskaltsis, Angela
AU - Sinclair, Nicholas R.Stc
PY - 1990/5/1
Y1 - 1990/5/1
N2 - Non-autoimmune prone CBA mice were compared with autoimmune prone NZB, NZW, and (NZB × NZW)F1 mice for the ability of their splenic cells to produce anti-ssDNA-forming cells spontaneously in vitro, measured in the plaque forming cell assay. The number of antibody forming cells was measured and the relative avidity of antibody produced determined using a plaque inhibition assay. Splenic lymphocytes from young animals of a non-autoimmune strain (CBAIJ) were shown to be capable of generating anti-ssDNA IgM antibody-forming cells in culture which displayed a higher avidity for antigen than that from autoimmune-prone or frankly autoimmune mice. Since an increased switching from IgM to IgG autoantibody production and defects in Fc-mediated signalling by IgG antibody have been identified in autoimmunity, we suggest that the metabolic block, normally in force in non-autoimmune-prone animals, accounts for this elevated avidity of IgM autoantibody.
AB - Non-autoimmune prone CBA mice were compared with autoimmune prone NZB, NZW, and (NZB × NZW)F1 mice for the ability of their splenic cells to produce anti-ssDNA-forming cells spontaneously in vitro, measured in the plaque forming cell assay. The number of antibody forming cells was measured and the relative avidity of antibody produced determined using a plaque inhibition assay. Splenic lymphocytes from young animals of a non-autoimmune strain (CBAIJ) were shown to be capable of generating anti-ssDNA IgM antibody-forming cells in culture which displayed a higher avidity for antigen than that from autoimmune-prone or frankly autoimmune mice. Since an increased switching from IgM to IgG autoantibody production and defects in Fc-mediated signalling by IgG antibody have been identified in autoimmunity, we suggest that the metabolic block, normally in force in non-autoimmune-prone animals, accounts for this elevated avidity of IgM autoantibody.
KW - End product feedback
KW - Immunoregulation
KW - Regulation by Fc signals
KW - Regulation of antibody responses
KW - Self-non-self discrimination
UR - http://www.scopus.com/inward/record.url?scp=0025313753&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0025313753&partnerID=8YFLogxK
U2 - 10.1093/intimm/2.5.381
DO - 10.1093/intimm/2.5.381
M3 - Article
C2 - 2085483
AN - SCOPUS:0025313753
VL - 2
SP - 381
EP - 389
JO - International Immunology
JF - International Immunology
SN - 0953-8178
IS - 5
ER -