Abstract
A number of small circular DNAs constitute a part of the genome of Entamoeba histolytica. Among them, the 24.5kb circular DNA encoding rRNA (EhR1) is the most abundant. Pulsed field gel electrophoresis was used to determine if a chromosomal copy of EhR1 exists and what fraction of the total genome is circular. The results show that the chromosomes of E. histolytica are linear, and that no copy of EhR1 could be detected in any of the linear chromosomes. Copyright (C) 1999 Australian Society for Parasitology Inc.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1775-1783 |
| Number of pages | 9 |
| Journal | International Journal for Parasitology |
| Volume | 29 |
| Issue number | 11 |
| DOIs | |
| State | Published - Nov 1999 |
| Externally published | Yes |
Bibliographical note
Funding Information:A. Bagchi thanks UGC for Research Fellowship. This work was supported by a grant from DST, Government of India.
Keywords
- Anomalous migration
- Circular DNA
- Entamoeba histolytica
- Exonuclease Bal31
- Extrachromosomal rDNA
- Genome mapping
- Molecular karyotype
- Pulsed field gel electrophoresis
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