Label-free impedimetric sensing of cortisol in human serum based on nanowell array electrodes

Seyed Reza Mahmoodi, Pengfei Xie, Daniel P. Zachs, Erik J. Peterson, Hubert H. Lim, Mark Allen, Mehdi Javanmard

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

This study presents a real-time impedimetric biosensor to detect cortisol in human serum in small volumes in the range of 1-20 µL. Continuous monitoring of cortisol is closely related to maintaining healthy physiological conditions. A 28-well plate biosensor chip was built after several steps of fabrication. The achieved nanowell array structure provides enhanced electrochemical sensitivity which enables to detect cortisol at high salt concentration of human serum. Human serum samples were collected to compare the sensor response with results from the ELISA method. The robust performance of the device was validated from the proportional response of both measurements.

Original languageEnglish (US)
Title of host publicationMicroTAS 2020 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PublisherChemical and Biological Microsystems Society
Pages526-527
Number of pages2
ISBN (Electronic)9781733419017
StatePublished - 2020
Event24th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2020 - Virtual, Online
Duration: Oct 4 2020Oct 9 2020

Publication series

NameMicroTAS 2020 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences

Conference

Conference24th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2020
CityVirtual, Online
Period10/4/2010/9/20

Bibliographical note

Funding Information:
This work was sponsored by the Defense Advanced Research Projects Agency (DARPA) Biological Technologies Office (BTO) Electrical Prescriptions (ElectRx) program managed by Dr. E. V. Giesen through the DARPA Contracts Management Office Grant/Contract No HR0011-16-2-0026.

Publisher Copyright:
© 2020 CBMS-0001

Keywords

  • Biosensor
  • Cortisol
  • Impedance
  • Nanowell

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