Kruppel-like factor 2 is required for trafficking but not quiescence in postactivated T cells

Kensuke Takada, Xiaodan Wang, Geoffrey T. Hart, Oludare A. Odumade, Michael A. Weinreich, Kristin A. Hogquist, Stephen C. Jameson

Research output: Contribution to journalArticlepeer-review

35 Scopus citations

Abstract

The transcription factor Kruppel-like factor 2 (KLF2) was proposed to regulate genes involved in cell cycle entry and T cell trafficking; however, the physiological role of its expression in postactivated T cells is not well defined. Previous studies suggested that the cytokines IL-2 and IL-15 differentially regulate KLF2 re-expression in postactivation T cells and that these cytokines also influence effector versus memory T cell differentiation. Using conditional and inducible KLF2-knockout model systems, we tested the specific role of KLF2 expression in activated CD8+ T cells cultured with these cytokines. KLF2 was required for effective transcription of sphingosine-1-phosphate receptor-1 (S1P1) and CD62L in postactivation T cells. However, although different cytokines dramatically altered the expression of cell-cycle-related genes, endogenous KLF2 had a minimal impact. Correspondingly, KLF2-deficient T cells showed dysregulated trafficking but not altered proliferative characteristics following in vivo responses to Ag. Thus, our data help to define KLF2-dependent and -independent aspects of activatedCD8 + T cell differentiation and argue against a physiological role in cell cycle regulation.

Original languageEnglish (US)
Pages (from-to)775-783
Number of pages9
JournalJournal of Immunology
Volume186
Issue number2
DOIs
StatePublished - Jan 15 2011

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