Abstract
The kinetics of mercuric ion (Hg2+) reduction by Escherichia coli was studied with ether-permeabilized and intact cells. Permeabilized cells reduced Hg2+ 5.5 times faster than intact cells. The reduction by intact cells exhibited Michaelis-Menten kinetic behavior with respect to Hg2+ concentration. In contrast, the reduction by permeabilized cells followed substrate inhibition kinetics with respect to Hg2+ concentration and Michaelis-Menten kinetics with respect to NADPH concentration. An expression was adopted to describe the simultaneous dependence of the reduction rate of permeabilized cells on the Hg2+ and NADPH concentrations. Experimental data confirmed the general validity of the rate expression in a wide range of Hg2+ and NADPH concentrations.
Original language | English (US) |
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Pages (from-to) | 854-859 |
Number of pages | 6 |
Journal | Enzyme and Microbial Technology |
Volume | 12 |
Issue number | 11 |
DOIs | |
State | Published - Nov 1990 |
Bibliographical note
Funding Information:This work was supported in part by grants from Eco-lab Inc., St Paul, Minnesota, the National Science Foundation (ECE-8552670), and the Graduate School of the University of Minnesota.
Keywords
- Mercuric reduction
- permeabilization
- substrate inhibition