A hybridoma cell line, AFP-27, was cultivated in a stirred tank with continuous perfusion. Microfiltration membrane was used to partially retain the cells. Using off-line measurement of glucose concentration the perfusion rate was increased in steps with increasing cell concentration. The specific rates of growth, glucose consumption, and antibody production appear to decrease with time. The need for kinetic models for optimization is discussed.
Bibliographical noteFunding Information:
This work was supported in part by grants from Ecolab, Inc. (St. Paul, MN) and the National Science Foundation (ECE-8512427 and ECE-8552670). We thank W. R. Grace & Co. for providing us with the media.