Kinetics of growth and antibody production by a hybridoma cell line in a perfusion culture

T. Craig Seamans, Wei Shou Hu

Research output: Contribution to journalArticlepeer-review

40 Scopus citations

Abstract

A hybridoma cell line, AFP-27, was cultivated in a stirred tank with continuous perfusion. Microfiltration membrane was used to partially retain the cells. Using off-line measurement of glucose concentration the perfusion rate was increased in steps with increasing cell concentration. The specific rates of growth, glucose consumption, and antibody production appear to decrease with time. The need for kinetic models for optimization is discussed.

Original languageEnglish (US)
Pages (from-to)241-245
Number of pages5
JournalJournal of Fermentation and Bioengineering
Volume70
Issue number4
DOIs
StatePublished - 1990

Bibliographical note

Funding Information:
This work was supported in part by grants from Ecolab, Inc. (St. Paul, MN) and the National Science Foundation (ECE-8512427 and ECE-8552670). We thank W. R. Grace & Co. for providing us with the media.

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