TY - JOUR
T1 - Kinetics of gossypol inhibition of bovine lactate dehydrogenase X
AU - Olgiati, Katherine L.
AU - Toscano, William A.
N1 - Copyright:
Copyright 2014 Elsevier B.V., All rights reserved.
PY - 1983/8/30
Y1 - 1983/8/30
N2 - Gossypol, a polyphenolic binaphthalene dialdehyde isolated from cotton meal is a potent inhibitor of lactate dehydrogenase-X purified from bovine testis. For the conversion of pyruvate to lactate the IC50 for gossypol is 200 μM for the reverse reaction the IC50 is 12 μM. Gossypol is a competitive inhibitor of NADH, Ki = 30 μM (Km = 17 μM), and NAD+, Ki = 6 μM (Km = 130 μM), and noncompetitive for pyruvate, Ki = 220 μM (Km = 224 μM), and lactate, Ki = 52 μM (Km = 5.6 mM).
AB - Gossypol, a polyphenolic binaphthalene dialdehyde isolated from cotton meal is a potent inhibitor of lactate dehydrogenase-X purified from bovine testis. For the conversion of pyruvate to lactate the IC50 for gossypol is 200 μM for the reverse reaction the IC50 is 12 μM. Gossypol is a competitive inhibitor of NADH, Ki = 30 μM (Km = 17 μM), and NAD+, Ki = 6 μM (Km = 130 μM), and noncompetitive for pyruvate, Ki = 220 μM (Km = 224 μM), and lactate, Ki = 52 μM (Km = 5.6 mM).
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U2 - 10.1016/0006-291X(83)90986-5
DO - 10.1016/0006-291X(83)90986-5
M3 - Article
C2 - 6615526
AN - SCOPUS:0020561972
SN - 0006-291X
VL - 115
SP - 180
EP - 185
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 1
ER -