Kinetics of gossypol inhibition of bovine lactate dehydrogenase X

Katherine L. Olgiati, William A. Toscano

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Gossypol, a polyphenolic binaphthalene dialdehyde isolated from cotton meal is a potent inhibitor of lactate dehydrogenase-X purified from bovine testis. For the conversion of pyruvate to lactate the IC50 for gossypol is 200 μM for the reverse reaction the IC50 is 12 μM. Gossypol is a competitive inhibitor of NADH, Ki = 30 μM (Km = 17 μM), and NAD+, Ki = 6 μM (Km = 130 μM), and noncompetitive for pyruvate, Ki = 220 μM (Km = 224 μM), and lactate, Ki = 52 μM (Km = 5.6 mM).

Original languageEnglish (US)
Pages (from-to)180-185
Number of pages6
JournalBiochemical and Biophysical Research Communications
Issue number1
StatePublished - Aug 30 1983
Externally publishedYes

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