Kinetics of cytolytic T lymphocyte binding to target cells in suspension

S. P. Balk, J. Walker, Matthew F Mescher

Research output: Contribution to journalArticle

15 Scopus citations

Abstract

Cytolytic T lymphocytes (CTL) were able to specifically bind and lyse allogeneic P815 tumor cells and LPS blast cells in suspension. An assay was developed to measure the rate of target cell binding in suspension independent of the rate of lysis. Target cell binding was found to plateau within 3 hr in suspension. The presence of free, functional CTL and targets at these plateaus was demonstrated, indicating that target cell binding was an equilibrium process. Scatchard plots were used to derive values for Kd (apparent affinity) and bmax (maximum binding). Target cell binding was compared for CTL generated by secondary in vitro stimulation with intact cells or with purified membranes. These 2 CTL populations yielded distinct values for Kd and bmax. Implications of this kinetic difference for CTL recogniton of purified plasma membranes are discussed.

Original languageEnglish (US)
Pages (from-to)2177-2183
Number of pages7
JournalJournal of Immunology
Volume126
Issue number6
StatePublished - Jan 1 1981

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    Balk, S. P., Walker, J., & Mescher, M. F. (1981). Kinetics of cytolytic T lymphocyte binding to target cells in suspension. Journal of Immunology, 126(6), 2177-2183.