Kinetics model for the wavelength-dependence of excited-state dynamics of hetero-FRET sensors

Jacob Schwarz; Ryan Leighton; Hannah J. Leopold; Megan Currie; Arnold J. Boersma; Erin Sheets; Ahmed A Heikal

doi:10.1117/12.2274793

Kinetics model for the wavelength-dependence of excited-state dynamics of hetero-FRET sensors

Jacob Schwarz, Ryan Leighton, Hannah J. Leopold, Megan Currie, Arnold J. Boersma, Erin Sheets, Ahmed A Heikal

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

4 Scopus citations

Abstract

Foerster (or fluorescence) resonance energy transfer (FRET) is a powerful tool for investigating protein-protein interactions, in both living cells and in controlled environments. A typical hetero-FRET pair consists of a donor and acceptor tethered together with a linker. The corresponding energy transfer efficiency of a hetero-FRET pair probe depends upon the donor-acceptor distance, relative dipole orientation, and spectral overlap. Because of the sensitivity of the energy transfer efficiency on the donor-acceptor distance, FRET is often referred to as a "molecular ruler". Time-resolved fluorescence approach for measuring the excited-state lifetime of the donor and acceptor emissions is one of the most reliable approaches for quantitative assessment of the energy transfer efficiency in hetero-FRET pairs. In this contribution, we provide an analytical kinetics model that describes the excited-state depopulation of a FRET probe as a means to predicts the time-resolved fluorescence profile as a function of excitation and detection wavelengths. In addition, we used this developed kinetics model to simulate the time-dependence of the excited-state population of both the donor and acceptor. These results should serve as a guide for our ongoing studies of newly developed hetero-FRET sensors (mCerulean3-linker-mCitrine) that are designed specifically for in vivo studies of macromolecular crowding. The same model is applicable to other FRET pairs with the careful consideration of their steady-state spectroscopy and the experimental design for wavelength- dependence of the fluorescence lifetime measurements.

Original language	English (US)
Title of host publication	Ultrafast Nonlinear Imaging and Spectroscopy V
Editors	Zhiwen Liu, Kebin Shi, Iam Choon Khoo, Demetri Psaltis
Publisher	SPIE
ISBN (Electronic)	9781510612174
DOIs	https://doi.org/10.1117/12.2274793
State	Published - 2017
Event	Ultrafast Nonlinear Imaging and Spectroscopy V 2017 - San Diego, United States Duration: Aug 6 2017 → Aug 7 2017

Publication series

Name	Proceedings of SPIE - The International Society for Optical Engineering
Volume	10380
ISSN (Print)	0277-786X
ISSN (Electronic)	1996-756X

Conference

Conference	Ultrafast Nonlinear Imaging and Spectroscopy V 2017
Country/Territory	United States
City	San Diego
Period	8/6/17 → 8/7/17

Bibliographical note

Funding Information:
E.D.S. and A.A.H. acknowledge the financial support provided by the University of Minnesota Grant-in-Aid. A.J.B. also acknowledges the financial support of the Netherlands Organization for Scientific Research Vidi grant. R.L. was supported by the Swenson Family Foundation. Additionally, the financial support provided by a Chancellor’s Small Grant, the Department of Chemistry and Biochemistry, the Swenson College of Science and Engineering, all of which are from the University of Minnesota Duluth. M.C., J.S. and H.L. were supported by teaching fellowships from the University of Minnesota Duluth Department of Chemistry and Biochemistry. The authors also acknowledge the Minnesota Supercomputing Institute (MSI) at the University of Minnesota for providing resources that contributed to the research results reported within this paper.

Publisher Copyright:
© 2017 SPIE.

Keywords

FRET
excited-state dynamics
fluorescence lifetime
kinetics model

Publisher link

10.1117/12.2274793

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Schwarz, J., Leighton, R., Leopold, H. J., Currie, M., Boersma, A. J., Sheets, E., & Heikal, A. A. (2017). Kinetics model for the wavelength-dependence of excited-state dynamics of hetero-FRET sensors. In Z. Liu, K. Shi, I. C. Khoo, & D. Psaltis (Eds.), Ultrafast Nonlinear Imaging and Spectroscopy V Article 103800S (Proceedings of SPIE - The International Society for Optical Engineering; Vol. 10380). SPIE. https://doi.org/10.1117/12.2274793

Kinetics model for the wavelength-dependence of excited-state dynamics of hetero-FRET sensors. / Schwarz, Jacob; Leighton, Ryan; Leopold, Hannah J. et al.
Ultrafast Nonlinear Imaging and Spectroscopy V. ed. / Zhiwen Liu; Kebin Shi; Iam Choon Khoo; Demetri Psaltis. SPIE, 2017. 103800S (Proceedings of SPIE - The International Society for Optical Engineering; Vol. 10380).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Schwarz, J, Leighton, R, Leopold, HJ, Currie, M, Boersma, AJ, Sheets, E & Heikal, AA 2017, Kinetics model for the wavelength-dependence of excited-state dynamics of hetero-FRET sensors. in Z Liu, K Shi, IC Khoo & D Psaltis (eds), Ultrafast Nonlinear Imaging and Spectroscopy V., 103800S, Proceedings of SPIE - The International Society for Optical Engineering, vol. 10380, SPIE, Ultrafast Nonlinear Imaging and Spectroscopy V 2017, San Diego, United States, 8/6/17. https://doi.org/10.1117/12.2274793

Schwarz J, Leighton R, Leopold HJ, Currie M, Boersma AJ, Sheets E et al. Kinetics model for the wavelength-dependence of excited-state dynamics of hetero-FRET sensors. In Liu Z, Shi K, Khoo IC, Psaltis D, editors, Ultrafast Nonlinear Imaging and Spectroscopy V. SPIE. 2017. 103800S. (Proceedings of SPIE - The International Society for Optical Engineering). doi: 10.1117/12.2274793

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title = "Kinetics model for the wavelength-dependence of excited-state dynamics of hetero-FRET sensors",

abstract = "Foerster (or fluorescence) resonance energy transfer (FRET) is a powerful tool for investigating protein-protein interactions, in both living cells and in controlled environments. A typical hetero-FRET pair consists of a donor and acceptor tethered together with a linker. The corresponding energy transfer efficiency of a hetero-FRET pair probe depends upon the donor-acceptor distance, relative dipole orientation, and spectral overlap. Because of the sensitivity of the energy transfer efficiency on the donor-acceptor distance, FRET is often referred to as a {"}molecular ruler{"}. Time-resolved fluorescence approach for measuring the excited-state lifetime of the donor and acceptor emissions is one of the most reliable approaches for quantitative assessment of the energy transfer efficiency in hetero-FRET pairs. In this contribution, we provide an analytical kinetics model that describes the excited-state depopulation of a FRET probe as a means to predicts the time-resolved fluorescence profile as a function of excitation and detection wavelengths. In addition, we used this developed kinetics model to simulate the time-dependence of the excited-state population of both the donor and acceptor. These results should serve as a guide for our ongoing studies of newly developed hetero-FRET sensors (mCerulean3-linker-mCitrine) that are designed specifically for in vivo studies of macromolecular crowding. The same model is applicable to other FRET pairs with the careful consideration of their steady-state spectroscopy and the experimental design for wavelength- dependence of the fluorescence lifetime measurements.",

keywords = "FRET, excited-state dynamics, fluorescence lifetime, kinetics model",

author = "Jacob Schwarz and Ryan Leighton and Leopold, {Hannah J.} and Megan Currie and Boersma, {Arnold J.} and Erin Sheets and Heikal, {Ahmed A}",

note = "Funding Information: E.D.S. and A.A.H. acknowledge the financial support provided by the University of Minnesota Grant-in-Aid. A.J.B. also acknowledges the financial support of the Netherlands Organization for Scientific Research Vidi grant. R.L. was supported by the Swenson Family Foundation. Additionally, the financial support provided by a Chancellor{\textquoteright}s Small Grant, the Department of Chemistry and Biochemistry, the Swenson College of Science and Engineering, all of which are from the University of Minnesota Duluth. M.C., J.S. and H.L. were supported by teaching fellowships from the University of Minnesota Duluth Department of Chemistry and Biochemistry. The authors also acknowledge the Minnesota Supercomputing Institute (MSI) at the University of Minnesota for providing resources that contributed to the research results reported within this paper. Publisher Copyright: {\textcopyright} 2017 SPIE.; Ultrafast Nonlinear Imaging and Spectroscopy V 2017 ; Conference date: 06-08-2017 Through 07-08-2017",

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TY - GEN

T1 - Kinetics model for the wavelength-dependence of excited-state dynamics of hetero-FRET sensors

AU - Schwarz, Jacob

AU - Leighton, Ryan

AU - Leopold, Hannah J.

AU - Currie, Megan

AU - Boersma, Arnold J.

AU - Sheets, Erin

AU - Heikal, Ahmed A

N1 - Funding Information: E.D.S. and A.A.H. acknowledge the financial support provided by the University of Minnesota Grant-in-Aid. A.J.B. also acknowledges the financial support of the Netherlands Organization for Scientific Research Vidi grant. R.L. was supported by the Swenson Family Foundation. Additionally, the financial support provided by a Chancellor’s Small Grant, the Department of Chemistry and Biochemistry, the Swenson College of Science and Engineering, all of which are from the University of Minnesota Duluth. M.C., J.S. and H.L. were supported by teaching fellowships from the University of Minnesota Duluth Department of Chemistry and Biochemistry. The authors also acknowledge the Minnesota Supercomputing Institute (MSI) at the University of Minnesota for providing resources that contributed to the research results reported within this paper. Publisher Copyright: © 2017 SPIE.

PY - 2017

Y1 - 2017

N2 - Foerster (or fluorescence) resonance energy transfer (FRET) is a powerful tool for investigating protein-protein interactions, in both living cells and in controlled environments. A typical hetero-FRET pair consists of a donor and acceptor tethered together with a linker. The corresponding energy transfer efficiency of a hetero-FRET pair probe depends upon the donor-acceptor distance, relative dipole orientation, and spectral overlap. Because of the sensitivity of the energy transfer efficiency on the donor-acceptor distance, FRET is often referred to as a "molecular ruler". Time-resolved fluorescence approach for measuring the excited-state lifetime of the donor and acceptor emissions is one of the most reliable approaches for quantitative assessment of the energy transfer efficiency in hetero-FRET pairs. In this contribution, we provide an analytical kinetics model that describes the excited-state depopulation of a FRET probe as a means to predicts the time-resolved fluorescence profile as a function of excitation and detection wavelengths. In addition, we used this developed kinetics model to simulate the time-dependence of the excited-state population of both the donor and acceptor. These results should serve as a guide for our ongoing studies of newly developed hetero-FRET sensors (mCerulean3-linker-mCitrine) that are designed specifically for in vivo studies of macromolecular crowding. The same model is applicable to other FRET pairs with the careful consideration of their steady-state spectroscopy and the experimental design for wavelength- dependence of the fluorescence lifetime measurements.

AB - Foerster (or fluorescence) resonance energy transfer (FRET) is a powerful tool for investigating protein-protein interactions, in both living cells and in controlled environments. A typical hetero-FRET pair consists of a donor and acceptor tethered together with a linker. The corresponding energy transfer efficiency of a hetero-FRET pair probe depends upon the donor-acceptor distance, relative dipole orientation, and spectral overlap. Because of the sensitivity of the energy transfer efficiency on the donor-acceptor distance, FRET is often referred to as a "molecular ruler". Time-resolved fluorescence approach for measuring the excited-state lifetime of the donor and acceptor emissions is one of the most reliable approaches for quantitative assessment of the energy transfer efficiency in hetero-FRET pairs. In this contribution, we provide an analytical kinetics model that describes the excited-state depopulation of a FRET probe as a means to predicts the time-resolved fluorescence profile as a function of excitation and detection wavelengths. In addition, we used this developed kinetics model to simulate the time-dependence of the excited-state population of both the donor and acceptor. These results should serve as a guide for our ongoing studies of newly developed hetero-FRET sensors (mCerulean3-linker-mCitrine) that are designed specifically for in vivo studies of macromolecular crowding. The same model is applicable to other FRET pairs with the careful consideration of their steady-state spectroscopy and the experimental design for wavelength- dependence of the fluorescence lifetime measurements.

KW - FRET

KW - excited-state dynamics

KW - fluorescence lifetime

KW - kinetics model

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M3 - Conference contribution

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T3 - Proceedings of SPIE - The International Society for Optical Engineering

BT - Ultrafast Nonlinear Imaging and Spectroscopy V

A2 - Liu, Zhiwen

A2 - Shi, Kebin

A2 - Khoo, Iam Choon

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PB - SPIE

T2 - Ultrafast Nonlinear Imaging and Spectroscopy V 2017

Y2 - 6 August 2017 through 7 August 2017

ER -

Kinetics model for the wavelength-dependence of excited-state dynamics of hetero-FRET sensors

Abstract

Publication series

Conference

Bibliographical note

Keywords

Publisher link

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