Abstract
The authors describe a rapid, accurate, and precise 2-point kinetic assay for ethylene glycol. The method involves use of a standard kit for ethanol determination with yeast alcohol dehydrogenase, and of a centrifugal analyzer. Alcohol dehydrogenase catalyzes the oxidation of ethylene glycol in a trichloroacetic acid-precipitated specimen; the resulting NADH production is monitored at 340 nm. The reaction rate is linear with concentration to 1.5 g of ethylene glycol per liter. Interference from methanol, ethanol, and isopropanol was easily recognized after a 30-min incubation at 100°C. The authors believe that the method can be readily adaptable to any narrow-bandwith, stable, temperature-controlled spectrophotometer and so should provide more widely for the prompt assessment of patients in whom ethylene glycol poisoning is suspected.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1278-1280 |
| Number of pages | 3 |
| Journal | Clinical chemistry |
| Volume | 26 |
| Issue number | 9 |
| DOIs | |
| State | Published - 1980 |