Kinetic ethylene glycol assay with use of yeast alcohol dehydrogenase

J. H. Eckfeldt, R. T. Light

Research output: Contribution to journalArticlepeer-review

16 Scopus citations

Abstract

The authors describe a rapid, accurate, and precise 2-point kinetic assay for ethylene glycol. The method involves use of a standard kit for ethanol determination with yeast alcohol dehydrogenase, and of a centrifugal analyzer. Alcohol dehydrogenase catalyzes the oxidation of ethylene glycol in a trichloroacetic acid-precipitated specimen; the resulting NADH production is monitored at 340 nm. The reaction rate is linear with concentration to 1.5 g of ethylene glycol per liter. Interference from methanol, ethanol, and isopropanol was easily recognized after a 30-min incubation at 100°C. The authors believe that the method can be readily adaptable to any narrow-bandwith, stable, temperature-controlled spectrophotometer and so should provide more widely for the prompt assessment of patients in whom ethylene glycol poisoning is suspected.

Original languageEnglish (US)
Pages (from-to)1278-1280
Number of pages3
JournalClinical chemistry
Volume26
Issue number9
DOIs
StatePublished - 1980

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