Kinetic enzymic assay for D(-)-lactate, with use of a centrifugal analyzer

C. W. Ludvigsen, J. R. Thurn, G. L. Pierpont, J. H. Eckfeldt

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22 Scopus citations

Abstract

The D(-) isomer of lactic acid appears to cause a form of metabolic encephalopathy experienced by patients who have jejunoileal bypass for morbid obesity. However, analysis for D(-)-lactate is not routinely available in clinical or reference laboratories. We describe an enzymic centrifugal-analyzer assay for D(-)-lactate in plasma or serum, with use of D(-)-lactate dehydrogenase. The method involves two-point kinetic calibration and preincubation of specimen and NAD+, thus eliminating the need for specimen-blanking or protein-precipitating pretreatment. This rapid, accurate, and precise assay should be helpful in evaluating patients with 'short-bowel syndrome' who display confusion, lethargy, ataxia, or other central nervous-system disturbances that may be ascribable to D(-)-lactic acidosis.

Original languageEnglish (US)
Pages (from-to)1823-1825
Number of pages3
JournalClinical chemistry
Volume29
Issue number10
DOIs
StatePublished - 1983

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