Kinetic Effects of Terbium Ions on Muscarinic Acetylcholine Receptors of Murine Neuroblastoma Cells

Esam E. El‐Fakahany, Michael Pfenning, Elliott Richelson

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

Abstract: Preincubation of murine neuroblastoma cells (clone NIE‐115) with terbium chloride resulted in a significant potentiation of carbachol‐mediated increase in cyclic GMP formation. This effect was accompanied by a shift of the peak response from 30 s to 120 s and a 6‐fold decrease in carbachol concentration producing half‐maximal responses, in addition to a significant increase in the Hill coefficient. Terbium ions also caused a significant decrease in the affinity and an increase in the maximum binding of [3H]quinuclidinyl benzilate to muscarinic receptors, the change in affinity being mainly due to a decrease in the association rate. Preincubation of cells with 1 mM carbachol for 4 h (the desensitized state of the muscarinic receptor) resulted in a decrease in the ability of terbium to alter [3H]quinuclidinyl benzilate binding. The effects of terbium reported here might be due to its affecting muscarinic receptor‐effector coupling, which is considered to be lost upon receptor desensitization.

Original languageEnglish (US)
Pages (from-to)863-869
Number of pages7
JournalJournal of Neurochemistry
Volume42
Issue number3
DOIs
StatePublished - Mar 1984

Keywords

  • Desensitization
  • Lanthanides
  • Murine neuroblastoma
  • Muscarinic receptors
  • Receptor‐effector coupling

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