Kinesins to the core: The role of microtubule-based motor proteins in building the mitotic spindle midzone

Jessica E. Hornick; Kul Karanjeet; Elizabeth S. Collins; Edward H Hinchcliffe

doi:10.1016/j.semcdb.2010.01.017

Kinesins to the core: The role of microtubule-based motor proteins in building the mitotic spindle midzone

Jessica E. Hornick, Kul Karanjeet, Elizabeth S. Collins, Edward H Hinchcliffe

Research output: Contribution to journal › Review article › peer-review

22 Scopus citations

Abstract

In mammalian cultured cells the initiation of cytokinesis is regulated - both temporally and spatially - by the overlapping, anti-parallel microtubules of the spindle midzone. This region recruits several key central spindle components: PRC-1, polo-like kinase 1 (Plk-1), the centralspindlin complex, and the chromosome passenger complex (CPC), which together serve to stabilize the microtubule overlap, and also to coordinate the assembly of the cortical actin/myosin cytoskeleton necessary to physically cleave the cell in two. The localization of these crucial elements to the spindle midzone requires members of the kinesin superfamily of microtubule-based motor proteins. Here we focus on reviewing the role played by a variety of kinesins in both building and operating the spindle midzone machinery during cytokinesis.

Original language	English (US)
Pages (from-to)	290-299
Number of pages	10
Journal	Seminars in Cell and Developmental Biology
Volume	21
Issue number	3
DOIs	https://doi.org/10.1016/j.semcdb.2010.01.017
State	Published - May 2010

Bibliographical note

Funding Information:
EHH wishes to thank Ron Vale for teaching him how to purify “real” kinesin (and calamari) during the 1992 MBL Physiology course in Woods Hole, MA. JEH is supported by a post-doctoral training grant from the National Institutes of Health ( T32 CA080621 ). Work in the Hinchcliffe lab is supported by a research grant from the National Institutes of Health ( R01 GM072754 ).

Keywords

Cytokinesis
Kinesin
Microtubule
Mitosis
Motor protein

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abstract = "In mammalian cultured cells the initiation of cytokinesis is regulated - both temporally and spatially - by the overlapping, anti-parallel microtubules of the spindle midzone. This region recruits several key central spindle components: PRC-1, polo-like kinase 1 (Plk-1), the centralspindlin complex, and the chromosome passenger complex (CPC), which together serve to stabilize the microtubule overlap, and also to coordinate the assembly of the cortical actin/myosin cytoskeleton necessary to physically cleave the cell in two. The localization of these crucial elements to the spindle midzone requires members of the kinesin superfamily of microtubule-based motor proteins. Here we focus on reviewing the role played by a variety of kinesins in both building and operating the spindle midzone machinery during cytokinesis.",

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author = "Hornick, {Jessica E.} and Kul Karanjeet and Collins, {Elizabeth S.} and Hinchcliffe, {Edward H}",

note = "Funding Information: EHH wishes to thank Ron Vale for teaching him how to purify “real” kinesin (and calamari) during the 1992 MBL Physiology course in Woods Hole, MA. JEH is supported by a post-doctoral training grant from the National Institutes of Health ( T32 CA080621 ). Work in the Hinchcliffe lab is supported by a research grant from the National Institutes of Health ( R01 GM072754 ).",

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AU - Collins, Elizabeth S.

AU - Hinchcliffe, Edward H

N1 - Funding Information: EHH wishes to thank Ron Vale for teaching him how to purify “real” kinesin (and calamari) during the 1992 MBL Physiology course in Woods Hole, MA. JEH is supported by a post-doctoral training grant from the National Institutes of Health ( T32 CA080621 ). Work in the Hinchcliffe lab is supported by a research grant from the National Institutes of Health ( R01 GM072754 ).

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N2 - In mammalian cultured cells the initiation of cytokinesis is regulated - both temporally and spatially - by the overlapping, anti-parallel microtubules of the spindle midzone. This region recruits several key central spindle components: PRC-1, polo-like kinase 1 (Plk-1), the centralspindlin complex, and the chromosome passenger complex (CPC), which together serve to stabilize the microtubule overlap, and also to coordinate the assembly of the cortical actin/myosin cytoskeleton necessary to physically cleave the cell in two. The localization of these crucial elements to the spindle midzone requires members of the kinesin superfamily of microtubule-based motor proteins. Here we focus on reviewing the role played by a variety of kinesins in both building and operating the spindle midzone machinery during cytokinesis.

AB - In mammalian cultured cells the initiation of cytokinesis is regulated - both temporally and spatially - by the overlapping, anti-parallel microtubules of the spindle midzone. This region recruits several key central spindle components: PRC-1, polo-like kinase 1 (Plk-1), the centralspindlin complex, and the chromosome passenger complex (CPC), which together serve to stabilize the microtubule overlap, and also to coordinate the assembly of the cortical actin/myosin cytoskeleton necessary to physically cleave the cell in two. The localization of these crucial elements to the spindle midzone requires members of the kinesin superfamily of microtubule-based motor proteins. Here we focus on reviewing the role played by a variety of kinesins in both building and operating the spindle midzone machinery during cytokinesis.

KW - Cytokinesis

KW - Kinesin

KW - Microtubule

KW - Mitosis

KW - Motor protein

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Kinesins to the core: The role of microtubule-based motor proteins in building the mitotic spindle midzone

Abstract

Bibliographical note

Keywords

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