Kenyan isolates of Puccinia graminis f. sp. tritici from 2008 to 2014: Virulence to SrTmp in the Ug99 race group and implications for breeding programs

Maria Newcomb; Pablo D. Olivera; Matthew N. Rouse; Les J. Szabo; Jerry Johnson; Sam Gale; Douglas G. Luster; Ruth Wanyera; Godwin Macharia; Sridhar Bhavani; David Hodson; Mehran Patpour; Mogens S. Hovmøller; Thomas G. Fetch; Yue Jin

doi:10.1094/PHYTO-12-15-0337-R

Kenyan isolates of Puccinia graminis f. sp. tritici from 2008 to 2014: Virulence to SrTmp in the Ug99 race group and implications for breeding programs

Maria Newcomb, Pablo D. Olivera, Matthew N. Rouse, Les J. Szabo, Jerry Johnson, Sam Gale, Douglas G. Luster, Ruth Wanyera, Godwin Macharia, Sridhar Bhavani, David Hodson, Mehran Patpour, Mogens S. Hovmøller, Thomas G. Fetch, Yue Jin

Plant Pathology

Research output: Contribution to journal › Article › peer-review

56 Scopus citations

Abstract

Frequent emergence of new variants in the Puccinia graminis f. sp. tritici Ug99 race group in Kenya has made pathogen survey a priority. We analyzed 140 isolates from 78 P. graminis f. sp. tritici samples collected in Kenya between 2008 and 2014 and identified six races, including three not detected prior to 2013. Genotypic analysis of 20 isolates from 2013 and 2014 collections showed that the new races TTHST, TTKTK, and TTKTT belong to the Ug99 race group. International advanced breeding lines were evaluated against an isolate of TTKTT (Sr31, Sr24, and SrTmp virulence) at the seedling stage. From 169 advanced lines from Kenya, 23% of lines with resistance to races TTKSK and TTKST were susceptible to TTKTT and, from two North American regional nurseries, 44 and 91% of resistant lines were susceptible. Three lines with combined resistance genes were developed to facilitate pathogen monitoring and race identification. These results indicate the increasing virulence and variability in the Kenyan P. graminis f. sp. tritici population and reveal vulnerabilities of elite germplasm to new races.

Original language	English (US)
Pages (from-to)	729-736
Number of pages	8
Journal	Phytopathology
Volume	106
Issue number	7
DOIs	https://doi.org/10.1094/PHYTO-12-15-0337-R
State	Published - Jul 2016

Bibliographical note

Funding Information:
This research received support from the Durable Rust Resistance in Wheat project administrated by Cornell University and funded by the Bill and Melinda Gates Foundation and the United Kingdom Department for International Development, and from the USDA-ARS National Plant Disease Recovery Plan. We thank S. Stoxen, L. Wanschura, M. Carter, and the KALRO pathology team of H. Wanga, P. Kinyanjui, F. Muchika, E. Githinji, L. Wolukao, V. M. Onyango, and E. O. Okello for their technical contributions. Mention of trademark, proprietary product, or vendor does not constitute a guarantee or warranty of the product by the USDA, and does not imply its approval to the exclusion of other products and vendors that might also be suitable.

Publisher Copyright:
© 2016, American Phytopathological Society. All rights reserved.

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10.1094/PHYTO-12-15-0337-R

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Newcomb, M., Olivera, P. D., Rouse, M. N., Szabo, L. J., Johnson, J., Gale, S., Luster, D. G., Wanyera, R., Macharia, G., Bhavani, S., Hodson, D., Patpour, M., Hovmøller, M. S., Fetch, T. G., & Jin, Y. (2016). Kenyan isolates of Puccinia graminis f. sp. tritici from 2008 to 2014: Virulence to SrTmp in the Ug99 race group and implications for breeding programs. Phytopathology, 106(7), 729-736. https://doi.org/10.1094/PHYTO-12-15-0337-R

Newcomb, M, Olivera, PD , Rouse, MN, Szabo, LJ, Johnson, J, Gale, S, Luster, DG, Wanyera, R, Macharia, G, Bhavani, S, Hodson, D, Patpour, M, Hovmøller, MS, Fetch, TG & Jin, Y 2016, 'Kenyan isolates of Puccinia graminis f. sp. tritici from 2008 to 2014: Virulence to SrTmp in the Ug99 race group and implications for breeding programs', Phytopathology, vol. 106, no. 7, pp. 729-736. https://doi.org/10.1094/PHYTO-12-15-0337-R

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title = "Kenyan isolates of Puccinia graminis f. sp. tritici from 2008 to 2014: Virulence to SrTmp in the Ug99 race group and implications for breeding programs",

abstract = "Frequent emergence of new variants in the Puccinia graminis f. sp. tritici Ug99 race group in Kenya has made pathogen survey a priority. We analyzed 140 isolates from 78 P. graminis f. sp. tritici samples collected in Kenya between 2008 and 2014 and identified six races, including three not detected prior to 2013. Genotypic analysis of 20 isolates from 2013 and 2014 collections showed that the new races TTHST, TTKTK, and TTKTT belong to the Ug99 race group. International advanced breeding lines were evaluated against an isolate of TTKTT (Sr31, Sr24, and SrTmp virulence) at the seedling stage. From 169 advanced lines from Kenya, 23% of lines with resistance to races TTKSK and TTKST were susceptible to TTKTT and, from two North American regional nurseries, 44 and 91% of resistant lines were susceptible. Three lines with combined resistance genes were developed to facilitate pathogen monitoring and race identification. These results indicate the increasing virulence and variability in the Kenyan P. graminis f. sp. tritici population and reveal vulnerabilities of elite germplasm to new races.",

author = "Maria Newcomb and Olivera, {Pablo D.} and Rouse, {Matthew N.} and Szabo, {Les J.} and Jerry Johnson and Sam Gale and Luster, {Douglas G.} and Ruth Wanyera and Godwin Macharia and Sridhar Bhavani and David Hodson and Mehran Patpour and Hovm{\o}ller, {Mogens S.} and Fetch, {Thomas G.} and Yue Jin",

note = "Funding Information: This research received support from the Durable Rust Resistance in Wheat project administrated by Cornell University and funded by the Bill and Melinda Gates Foundation and the United Kingdom Department for International Development, and from the USDA-ARS National Plant Disease Recovery Plan. We thank S. Stoxen, L. Wanschura, M. Carter, and the KALRO pathology team of H. Wanga, P. Kinyanjui, F. Muchika, E. Githinji, L. Wolukao, V. M. Onyango, and E. O. Okello for their technical contributions. Mention of trademark, proprietary product, or vendor does not constitute a guarantee or warranty of the product by the USDA, and does not imply its approval to the exclusion of other products and vendors that might also be suitable. Publisher Copyright: {\textcopyright} 2016, American Phytopathological Society. All rights reserved.",

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doi = "10.1094/PHYTO-12-15-0337-R",

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T1 - Kenyan isolates of Puccinia graminis f. sp. tritici from 2008 to 2014

T2 - Virulence to SrTmp in the Ug99 race group and implications for breeding programs

AU - Newcomb, Maria

AU - Olivera, Pablo D.

AU - Rouse, Matthew N.

AU - Szabo, Les J.

AU - Johnson, Jerry

AU - Gale, Sam

AU - Luster, Douglas G.

AU - Wanyera, Ruth

AU - Macharia, Godwin

AU - Bhavani, Sridhar

AU - Hodson, David

AU - Patpour, Mehran

AU - Hovmøller, Mogens S.

AU - Fetch, Thomas G.

AU - Jin, Yue

N1 - Funding Information: This research received support from the Durable Rust Resistance in Wheat project administrated by Cornell University and funded by the Bill and Melinda Gates Foundation and the United Kingdom Department for International Development, and from the USDA-ARS National Plant Disease Recovery Plan. We thank S. Stoxen, L. Wanschura, M. Carter, and the KALRO pathology team of H. Wanga, P. Kinyanjui, F. Muchika, E. Githinji, L. Wolukao, V. M. Onyango, and E. O. Okello for their technical contributions. Mention of trademark, proprietary product, or vendor does not constitute a guarantee or warranty of the product by the USDA, and does not imply its approval to the exclusion of other products and vendors that might also be suitable. Publisher Copyright: © 2016, American Phytopathological Society. All rights reserved.

PY - 2016/7

Y1 - 2016/7

N2 - Frequent emergence of new variants in the Puccinia graminis f. sp. tritici Ug99 race group in Kenya has made pathogen survey a priority. We analyzed 140 isolates from 78 P. graminis f. sp. tritici samples collected in Kenya between 2008 and 2014 and identified six races, including three not detected prior to 2013. Genotypic analysis of 20 isolates from 2013 and 2014 collections showed that the new races TTHST, TTKTK, and TTKTT belong to the Ug99 race group. International advanced breeding lines were evaluated against an isolate of TTKTT (Sr31, Sr24, and SrTmp virulence) at the seedling stage. From 169 advanced lines from Kenya, 23% of lines with resistance to races TTKSK and TTKST were susceptible to TTKTT and, from two North American regional nurseries, 44 and 91% of resistant lines were susceptible. Three lines with combined resistance genes were developed to facilitate pathogen monitoring and race identification. These results indicate the increasing virulence and variability in the Kenyan P. graminis f. sp. tritici population and reveal vulnerabilities of elite germplasm to new races.

AB - Frequent emergence of new variants in the Puccinia graminis f. sp. tritici Ug99 race group in Kenya has made pathogen survey a priority. We analyzed 140 isolates from 78 P. graminis f. sp. tritici samples collected in Kenya between 2008 and 2014 and identified six races, including three not detected prior to 2013. Genotypic analysis of 20 isolates from 2013 and 2014 collections showed that the new races TTHST, TTKTK, and TTKTT belong to the Ug99 race group. International advanced breeding lines were evaluated against an isolate of TTKTT (Sr31, Sr24, and SrTmp virulence) at the seedling stage. From 169 advanced lines from Kenya, 23% of lines with resistance to races TTKSK and TTKST were susceptible to TTKTT and, from two North American regional nurseries, 44 and 91% of resistant lines were susceptible. Three lines with combined resistance genes were developed to facilitate pathogen monitoring and race identification. These results indicate the increasing virulence and variability in the Kenyan P. graminis f. sp. tritici population and reveal vulnerabilities of elite germplasm to new races.

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Kenyan isolates of Puccinia graminis f. sp. tritici from 2008 to 2014: Virulence to SrTmp in the Ug99 race group and implications for breeding programs

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