Kappa immunoglobulin promoters and enhancers display developmentally controlled interactions

Regan Fulton, Brian Van Ness

Research output: Contribution to journalArticlepeer-review

32 Scopus citations

Abstract

We have investigated the interaction of the kappa immunoglobulin light chain intron and 3′ enhancers with two different kappa promoters at distinct stages of B-cell development. We find that transiently transfected reporter gene constructs driven by either the kappa V-region promoter, or the kappa germline promoter, are controlled by the known enhancers of the locus in a developmentally regulated fashion. We have, however, observed differences in promoter activation by eachenhancer. Moreover, constructs controlled by a combination of both enhancers are synergistically activated at the B-cell and plasma cell stages as compared with constructs containing either enhancer alone. This synergy is not observed early in development, at the pre-B cell stage. The pattern of enhancer and promoter interactions is discussed in the context of theknown developmental regulation of the locus.

Original languageEnglish (US)
Pages (from-to)4941-4947
Number of pages7
JournalNucleic acids research
Volume21
Issue number21
DOIs
StatePublished - Oct 25 1993

Bibliographical note

Copyright:
Copyright 2010 Elsevier B.V., All rights reserved.

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