Kainate receptor-mediated synaptic currents in mudpuppy inner retinal neurons reduced by D-O-phosphoserine

P. A. Coleman, R. F. Miller

Research output: Contribution to journalArticlepeer-review

9 Scopus citations

Abstract

The effects of D-O-phosphoserine (DOS) were examined on proximal neurons in the superfused mudpuppy retinal-eyecup preparation by measuring their synaptically evoked whole-cell currents with the use of patch-clamp electrodes. DOS reduced the light-evoked excitatory postsynaptic potentials (EPSPs) of amacrine and ganglion cells. This suppression was present even though the center responses of both ON- and OFF-bipolar cells were unaffected by DOS. When recordings were done under voltage-clamp conditions, DOS diminished the magnitude of light-evoked synaptic currents associated with a reduction in synaptic conductance. To determine which acidic amino acid receptor mediated the network-selective action of DOS, various glutamate agonists were tested against this excitatory amino acid receptor (EAAR) antagonist. DOS blocked the depolarizing effects of kainate (KA), but not those of N-methyl-D-aspartate (NMDA) or quisqualate (QQ). Thus DOS was a selective KA antagonist, and KA receptors appear to be the dominant EAAR subtype that mediates synaptic inputs into the inner retina of the mudpuppy.

Original languageEnglish (US)
Pages (from-to)495-500
Number of pages6
JournalJournal of neurophysiology
Volume62
Issue number2
DOIs
StatePublished - 1989

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