Ixodes ovatus ehrlichia exhibits unique ultrastructural characteristics in mammalian endothelial and tick-derived cells

Ulrike G Munderloh, David J. Silverman, Katherine C. MacNamara, Gilbert G. Ahlstrand, Madhumouli Chatterjee, Gary M. Winslow

Research output: Chapter in Book/Report/Conference proceedingChapter

11 Citations (Scopus)

Abstract

Tick-borne pathogens in the genus Ehrlichia cause emerging zoonoses. Although laboratory mice are susceptible to Ehrlichia infections, many isolates do not cause clinical illness. In contrast, the Ixodes ovatus Ehrlichia-like agent (IOE) causes disease and immune responses in mice comparable to the human illness caused by Ehrlichia chaffeensis. No culture system had been developed for IOE, however, which limited studies of this pathogen. We reasoned that endothelial and tick cell lines could potentially serve as host cells, since the IOE is found in ticks and in endothelial cells in mice. Infected spleen cells from RAG-deficient mice were overlaid onto ISE6 and RF6A cultures, and colonies typical of Ehrlichia were noted in RF6A cells within 2 weeks. Infection of ISE6 cells was established after transfer of IOE from RF6A cells. Electron microscopy revealed densely packed inclusions in infected RF6A and ISE6 cells; these inclusions contained copious amounts of filamentous structures, apparently originating from Ehrlichial cells. In particular, within RF6A cells the structures assumed an ordered morphology of finely combed hair. IOE from RF6A cells, when inoculated into C57BL6 and RAG-deficient mice, induced fatal disease. These data reveal unique structural features of IOE that may contribute to the pathogen's high virulence.

Original languageEnglish (US)
Title of host publicationRickettsiology and Rickettsial Diseases Fifth International Conference
PublisherBlackwell Publishing Inc
Pages112-119
Number of pages8
ISBN (Print)9781573317504
DOIs
StatePublished - Jan 1 2009

Publication series

NameAnnals of the New York Academy of Sciences
Volume1166
ISSN (Print)0077-8923
ISSN (Electronic)1749-6632

Fingerprint

Ehrlichia
Ixodes
Ticks
Pathogens
Endothelial cells
Ehrlichia chaffeensis
Endothelial Cells
Electron microscopy
Cells
Immune System Diseases
Zoonoses
Infection
Hair
Virulence
Electron Microscopy
Spleen

Keywords

  • Bacterial infection
  • Ehrlichia
  • Endothelial cell
  • Tick cell
  • Ultrastructure

Cite this

Munderloh, U. G., Silverman, D. J., MacNamara, K. C., Ahlstrand, G. G., Chatterjee, M., & Winslow, G. M. (2009). Ixodes ovatus ehrlichia exhibits unique ultrastructural characteristics in mammalian endothelial and tick-derived cells. In Rickettsiology and Rickettsial Diseases Fifth International Conference (pp. 112-119). (Annals of the New York Academy of Sciences; Vol. 1166). Blackwell Publishing Inc. https://doi.org/10.1111/j.1749-6632.2009.04520.x

Ixodes ovatus ehrlichia exhibits unique ultrastructural characteristics in mammalian endothelial and tick-derived cells. / Munderloh, Ulrike G; Silverman, David J.; MacNamara, Katherine C.; Ahlstrand, Gilbert G.; Chatterjee, Madhumouli; Winslow, Gary M.

Rickettsiology and Rickettsial Diseases Fifth International Conference. Blackwell Publishing Inc, 2009. p. 112-119 (Annals of the New York Academy of Sciences; Vol. 1166).

Research output: Chapter in Book/Report/Conference proceedingChapter

Munderloh, UG, Silverman, DJ, MacNamara, KC, Ahlstrand, GG, Chatterjee, M & Winslow, GM 2009, Ixodes ovatus ehrlichia exhibits unique ultrastructural characteristics in mammalian endothelial and tick-derived cells. in Rickettsiology and Rickettsial Diseases Fifth International Conference. Annals of the New York Academy of Sciences, vol. 1166, Blackwell Publishing Inc, pp. 112-119. https://doi.org/10.1111/j.1749-6632.2009.04520.x
Munderloh UG, Silverman DJ, MacNamara KC, Ahlstrand GG, Chatterjee M, Winslow GM. Ixodes ovatus ehrlichia exhibits unique ultrastructural characteristics in mammalian endothelial and tick-derived cells. In Rickettsiology and Rickettsial Diseases Fifth International Conference. Blackwell Publishing Inc. 2009. p. 112-119. (Annals of the New York Academy of Sciences). https://doi.org/10.1111/j.1749-6632.2009.04520.x
Munderloh, Ulrike G ; Silverman, David J. ; MacNamara, Katherine C. ; Ahlstrand, Gilbert G. ; Chatterjee, Madhumouli ; Winslow, Gary M. / Ixodes ovatus ehrlichia exhibits unique ultrastructural characteristics in mammalian endothelial and tick-derived cells. Rickettsiology and Rickettsial Diseases Fifth International Conference. Blackwell Publishing Inc, 2009. pp. 112-119 (Annals of the New York Academy of Sciences).
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