Isotope assay of histamine release

Malcolm N Blumenthal, Beatrice Roitman, Gary Carlson, Lloyd Fish

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

The single isotopic enzymatic histamine assay system has been adopted for the studying of the release of histamine from sensitized human leukocytes when challenged with the appropriate allergen. It is a simple, rapid, sensitive, and reproducible method based on the use of the enzyme histamine N-methyltransferase and the high specific activity of tritiated S adenosylmethionine.

Original languageEnglish (US)
Pages (from-to)312-317
Number of pages6
JournalBiochemical medicine
Volume11
Issue number4
DOIs
StatePublished - Dec 1974

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    Blumenthal, M. N., Roitman, B., Carlson, G., & Fish, L. (1974). Isotope assay of histamine release. Biochemical medicine, 11(4), 312-317. https://doi.org/10.1016/0006-2944(74)90129-X