The single isotopic enzymatic histamine assay system has been adopted for the studying of the release of histamine from sensitized human leukocytes when challenged with the appropriate allergen. It is a simple, rapid, sensitive, and reproducible method based on the use of the enzyme histamine N-methyltransferase and the high specific activity of tritiated S adenosylmethionine.
Bibliographical noteFunding Information:
1 This study was supported by funds of the Department of Minnesota Medical School and the Allergy Research Fund. 312 Copyright @ 1974 by Academic Press, Inc. All rights of reproduction in any form reserved.