TY - JOUR
T1 - Isolation of viable human muscle spindles for electron microscopic and physiologic study
AU - Kennedy, William R.
AU - Poppele, Richard E.
AU - Staley, Nancy A.
PY - 1974/8
Y1 - 1974/8
N2 - Human muscle spindles are isolated from muscle biopsies of Extensor Indicis. A spindle rich portion of muscle is removed, placed into a modified Kreb's solution and microdissected for spindles. Intramuscular nerves and blood vessels provide helpful locating guides. Spindles can be dissected free of extrafusal muscle with an adequate length of nerve for in vitro recording. Another portion of muscle is stretched and placed into a 5% glutaraldehyde fixative in 0.1 M cacodylate buffer. Spindles are dissected free, postfixed, dehydrated, stained, and then studied with the light microscope while in liquid Epon. The equatorial regions are identified, then removed and embedded for examination using the electron microscope. This is the first successful total isolation of viable human spindles. The combined procedures allow correlation of recordings from the primary sensory endings with the fine structure of spindles from normal persons and from patients with neuromuscular disorders.
AB - Human muscle spindles are isolated from muscle biopsies of Extensor Indicis. A spindle rich portion of muscle is removed, placed into a modified Kreb's solution and microdissected for spindles. Intramuscular nerves and blood vessels provide helpful locating guides. Spindles can be dissected free of extrafusal muscle with an adequate length of nerve for in vitro recording. Another portion of muscle is stretched and placed into a 5% glutaraldehyde fixative in 0.1 M cacodylate buffer. Spindles are dissected free, postfixed, dehydrated, stained, and then studied with the light microscope while in liquid Epon. The equatorial regions are identified, then removed and embedded for examination using the electron microscope. This is the first successful total isolation of viable human spindles. The combined procedures allow correlation of recordings from the primary sensory endings with the fine structure of spindles from normal persons and from patients with neuromuscular disorders.
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U2 - 10.1002/ar.1091790405
DO - 10.1002/ar.1091790405
M3 - Article
C2 - 4276391
AN - SCOPUS:0016302222
SN - 0003-276X
VL - 179
SP - 453
EP - 461
JO - The Anatomical Record
JF - The Anatomical Record
IS - 4
ER -