Isolation of selective 3H-chlornaltrexamine-bound complexes, possible opioid receptor components in brains of mice

Thomas P. Caruso, Dennis L. Larson, Philip S. Portoghese, A. E. Takemori

Research output: Contribution to journalArticlepeer-review

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The nonequilibrium narcotic antagonist, chlornaltrexamine (CNA) was used to bind selectively and covalently pioid specific sites on brain membrane preparations. Selective binding of [3H]CNA occured with a saturation maximum of 185 fmol/mg protein. Bound [3H]CNA was extracted with Triton X-100, dialyzed against Brij 36T, precipitated with trichloroacetic acid and chromatographed on an ultrogel AcA 22 column. The elution profile suggests that this extract contains a minimum of four selective [3H]CNA complexes. At least two of these complexes migrate in a single large peak. Column calibration showed that this peak eluted at 590,000 daltons. One of these specific [3H]CNA complexes elutes at the elution volume of the column and is dialyzable. Finally, putative aggregate of these complexes elutes with the void volume.

Original languageEnglish (US)
Pages (from-to)2063-2069
Number of pages7
JournalLife Sciences
Issue number22
StatePublished - Dec 1 1980

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