Isolation of ribosomal subunits from cultured mosquito cells

Ann Marie Fallon

doi:10.1016/0020-1790(83)90046-X

Isolation of ribosomal subunits from cultured mosquito cells

Ann Marie Fallon

Entomology

Research output: Contribution to journal › Article › peer-review

13 Scopus citations

Abstract

With cultured mosquito cells derived from Aedes albopictus, standard methods for preparation of ribosomal subunits have proved to be unsatisfactory. A new method has been developed for isolation of ribosomal subunits after treatment of mosquito cells with puromycin in vivo. Our results suggest that this method may be applicable to a variety of eukaryotic cell types.

Original language	English (US)
Pages (from-to)	251-255
Number of pages	5
Journal	Insect Biochemistry
Volume	13
Issue number	3
DOIs	https://doi.org/10.1016/0020-1790(83)90046-X
State	Published - 1983

Bibliographical note

Funding Information:
Acknowledgements~This work was supported by Grant AI-11290 from the National Institute of Allergy and Infectious Diseases. A.M.F. was supported by an N.I.H. postdoctoral fellowship. Drs. V. STOLLARa nd S. GILLIES participated in helpful discussions during the course of this work.

Keywords

cultured mosquito cells
puromycin
ribosomal subunits

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10.1016/0020-1790(83)90046-X

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title = "Isolation of ribosomal subunits from cultured mosquito cells",

abstract = "With cultured mosquito cells derived from Aedes albopictus, standard methods for preparation of ribosomal subunits have proved to be unsatisfactory. A new method has been developed for isolation of ribosomal subunits after treatment of mosquito cells with puromycin in vivo. Our results suggest that this method may be applicable to a variety of eukaryotic cell types.",

keywords = "cultured mosquito cells, puromycin, ribosomal subunits",

author = "Fallon, {Ann Marie}",

note = "Funding Information: Acknowledgements~This work was supported by Grant AI-11290 from the National Institute of Allergy and Infectious Diseases. A.M.F. was supported by an N.I.H. postdoctoral fellowship. Drs. V. STOLLARa nd S. GILLIES participated in helpful discussions during the course of this work.",

year = "1983",

doi = "10.1016/0020-1790(83)90046-X",

language = "English (US)",

volume = "13",

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T1 - Isolation of ribosomal subunits from cultured mosquito cells

AU - Fallon, Ann Marie

N1 - Funding Information: Acknowledgements~This work was supported by Grant AI-11290 from the National Institute of Allergy and Infectious Diseases. A.M.F. was supported by an N.I.H. postdoctoral fellowship. Drs. V. STOLLARa nd S. GILLIES participated in helpful discussions during the course of this work.

PY - 1983

Y1 - 1983

N2 - With cultured mosquito cells derived from Aedes albopictus, standard methods for preparation of ribosomal subunits have proved to be unsatisfactory. A new method has been developed for isolation of ribosomal subunits after treatment of mosquito cells with puromycin in vivo. Our results suggest that this method may be applicable to a variety of eukaryotic cell types.

AB - With cultured mosquito cells derived from Aedes albopictus, standard methods for preparation of ribosomal subunits have proved to be unsatisfactory. A new method has been developed for isolation of ribosomal subunits after treatment of mosquito cells with puromycin in vivo. Our results suggest that this method may be applicable to a variety of eukaryotic cell types.

KW - cultured mosquito cells

KW - puromycin

KW - ribosomal subunits

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JO - Insect Biochemistry and Molecular Biology

JF - Insect Biochemistry and Molecular Biology

IS - 3

ER -

Isolation of ribosomal subunits from cultured mosquito cells

Abstract

Bibliographical note

Keywords

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