Isolation of ribosomal subunits from cultured mosquito cells

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13 Scopus citations


With cultured mosquito cells derived from Aedes albopictus, standard methods for preparation of ribosomal subunits have proved to be unsatisfactory. A new method has been developed for isolation of ribosomal subunits after treatment of mosquito cells with puromycin in vivo. Our results suggest that this method may be applicable to a variety of eukaryotic cell types.

Original languageEnglish (US)
Pages (from-to)251-255
Number of pages5
JournalInsect Biochemistry
Issue number3
StatePublished - 1983

Bibliographical note

Funding Information:
Acknowledgements~This work was supported by Grant AI-11290 from the National Institute of Allergy and Infectious Diseases. A.M.F. was supported by an N.I.H. postdoctoral fellowship. Drs. V. STOLLARa nd S. GILLIES participated in helpful discussions during the course of this work.


  • cultured mosquito cells
  • puromycin
  • ribosomal subunits


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