Isolation of ribosomal subunits from cultured mosquito cells

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13 Scopus citations

Abstract

With cultured mosquito cells derived from Aedes albopictus, standard methods for preparation of ribosomal subunits have proved to be unsatisfactory. A new method has been developed for isolation of ribosomal subunits after treatment of mosquito cells with puromycin in vivo. Our results suggest that this method may be applicable to a variety of eukaryotic cell types.

Original languageEnglish (US)
Pages (from-to)251-255
Number of pages5
JournalInsect Biochemistry
Volume13
Issue number3
DOIs
StatePublished - 1983

Keywords

  • cultured mosquito cells
  • puromycin
  • ribosomal subunits

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