Isolation of replication-competent molecular clones of visna virus

Katherine A. Staskus, Ernest F. Retzel, Elizabeth D. Lewis, Janet L. Silsby, S. T. Sheila Cyr, Jeffrey M. Rank, Steven W. Wietgrefe, Ashley T. Haase, Ronald Cook, David Fast, Paul T. Geiser, John T. Harty, Selene H. Kong, Carol J. Lahti, Thomas P. Neufeld, Thomas E. Porter, Elizabeth Shoop, Karen R. Zachow

Research output: Contribution to journalArticle

55 Scopus citations

Abstract

Visna virus is the prototypic member of a subfamily of retroviruses responsible for slow infections of animals and humans. As a part of our investigation of the functions of viral gene products in virus replication, we have isolated three infectious molecular clones and determined the complete nucleotide sequences of two of the clones. We have also characterized the progeny of the biologically cloned viral stocks and of the infectious clones and document considerable heterogeneity in plaque size and antigenic phenotype of the former that is reduced to near homogeneity in the progeny of the infectious clones. It thus should now be possible to trace the emergence of antigenic variants of visna virus as well as ascribe defined functions to structural and regulatory genes of the virus in determining neurovirulence and the slow tempo of infection.

Original languageEnglish (US)
Pages (from-to)228-240
Number of pages13
JournalVirology
Volume181
Issue number1
DOIs
StatePublished - Mar 1991

Fingerprint Dive into the research topics of 'Isolation of replication-competent molecular clones of visna virus'. Together they form a unique fingerprint.

  • Cite this

    Staskus, K. A., Retzel, E. F., Lewis, E. D., Silsby, J. L., Sheila Cyr, S. T., Rank, J. M., Wietgrefe, S. W., Haase, A. T., Cook, R., Fast, D., Geiser, P. T., Harty, J. T., Kong, S. H., Lahti, C. J., Neufeld, T. P., Porter, T. E., Shoop, E., & Zachow, K. R. (1991). Isolation of replication-competent molecular clones of visna virus. Virology, 181(1), 228-240. https://doi.org/10.1016/0042-6822(91)90488-W