Isolation of oligotrophic denitrifiers carrying previously uncharacterized functional gene sequences

Satoshi Ishii, Naoaki Ashida, Shigeto Otsuka, Keishi Senoo

Research output: Contribution to journalArticlepeer-review

40 Scopus citations

Abstract

Oligotrophic denitrifying bacteria, including those belonging to the genera Herbaspirillum, Azospirillum, and Bradyrhizobium, were obtained using a single-cell isolation technique. The taxonomic composition of the denitrifier population was similar to those assessed by previous culture-independent studies. The sequencing of nitrite reductase and N2O reductase genes of these strains revealed previously unknown links between 16S rRNA and the denitrification-functional gene phylogenies. In particular, we identified Bradyrhizobium strains that harbor nirS sequences previously detected only in culture-independent studies.

Original languageEnglish (US)
Pages (from-to)338-342
Number of pages5
JournalApplied and environmental microbiology
Volume77
Issue number1
DOIs
StatePublished - Jan 2011

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