Isolation of nitric oxide synthase in the rat uterus

Yin Jaing, Ashok K Singh, Mathur S Kannan, Dana E Johnson, Ronald L. Shew

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Nitric oxide synthase (NOS) was isolated from the uterus of adult female rats by diethylaminoethyl (DEAE) column and further purified by 2',5'-ADP agarose. The chromatographic properties revealed two isoenzymes, NOS1 and NOS2. The molecular weights of both isoenzymes was approximately 155 Kd by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS PAGE) which was similar to NOS1 and NOS2 from rat brain. The enzymes required nicotinamide adenine dinucleotide phosphate (NADPH), Ca+2 and calmodulin as cofactors. However, in the absence of calmodulin and/or calcium NOS1 was reduced by approximately 96%, while NOS2 was reduced by approximately 70%. This maximal enzyme activity was similar for brain. These results demonstrate that two isoforms of NOS are present in the rat uterus.

Original languageEnglish (US)
Pages (from-to)1009-1014
Number of pages6
JournalLife Sciences
Volume58
Issue number12
DOIs
StatePublished - Feb 16 1996

Fingerprint

Nitric Oxide Synthase
Uterus
Rats
Calmodulin
Isoenzymes
Brain
Enzyme activity
Enzymes
Electrophoresis
NADP
Sodium Dodecyl Sulfate
Sepharose
Polyacrylamide Gel Electrophoresis
Protein Isoforms
Molecular Weight
Molecular weight
Calcium
polyacrylamide gels
adenosine 2',5'-diphosphate

Keywords

  • Female reproduction
  • Nitric oxide synthase
  • Sprague-Dawley rat
  • Uterus

Cite this

Isolation of nitric oxide synthase in the rat uterus. / Jaing, Yin; Singh, Ashok K; Kannan, Mathur S; Johnson, Dana E; Shew, Ronald L.

In: Life Sciences, Vol. 58, No. 12, 16.02.1996, p. 1009-1014.

Research output: Contribution to journalArticle

Jaing, Yin ; Singh, Ashok K ; Kannan, Mathur S ; Johnson, Dana E ; Shew, Ronald L. / Isolation of nitric oxide synthase in the rat uterus. In: Life Sciences. 1996 ; Vol. 58, No. 12. pp. 1009-1014.
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