Several studies have indicated the presence of microRNAs (miRNAs) within mitochondria, although the origin, as well as the biological function of these mitochondrially located miRNAs is largely unknown. The identifi cation and signifi cance of this subcellular localization is gaining increasing relevance to the pathogenesis of certain disease states. Here we describe the isolation of highly purifi ed mitochondria from rat liver by differential centrifugation, followed by RNAse A treatment to eliminate contaminating RNA. The coupled extraction of total RNA and protein is a more effi cient design for allowing the downstream evaluation of miRNA and protein expression in mitochondria.
|Original language||English (US)|
|Title of host publication||Mitochondrial Regulation|
|Subtitle of host publication||Methods and Protocols|
|Publisher||Springer New York|
|Number of pages||14|
|State||Published - Oct 11 2014|
- Differential centrifugation