Isolation of fibroblasts and epithelial cells in Bronchoalveolar Lavage (BAL)

Kathryn Pollock, Luke Albares, Chris Wendt, Allison Hubel

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

The long-term outcome of lung transplants is poor with 60%-70% of patients developing chronic rejection. Chronic rejection is manifested histologically by obliterative bronchiolitis with bronchiolitis obliterans syndrome (BOS), the clinical surrogate. Recent studies suggest that fibroblasts and epithelial cells present in bronchoalveolar lavage (BAL) may be a clinically relevant biomarker for BOS. The goal of this investigation was to develop a fast, repeatable method to individually isolate these low-frequency cell types. Fibroblasts and epithelial cells were isolated from BAL using attachment methods and the phenotype of the cells confirmed using immunostaining for vimentin (fibroblasts) and epithelial cell adhesion molecule (EpCAM, epithelial cells). Both fibroblasts and epithelial cells were isolated in every sample of BAL processed with the frequency of fibroblasts ranging from 0.03% to 0.48% and epithelial cells ranging from 0.05% to 1.5% of the total sample. Additional studies were performed using cytospins of cells after macrophages were depleted; cells exhibiting characteristics of both fibroblasts and epithelial cells were observed. The frequency of the cells of interest suggests that conventional methods of immunomagnetic isolation will not be effective in isolating these subpopulations. Finally, some of the low-frequency cells isolated via cytospin exhibit characteristics of epithelial to mesenchymal transition (which was not observed in plating incubations), indicating that the epithelial to mesenchymal cell transition fibroblasts may be nonadherent. In future studies, this technique and dataset may be of use to statistically correlate low-frequency cell type abundance to the onset and development of BOS.

Original languageEnglish (US)
Pages (from-to)146-154
Number of pages9
JournalExperimental Lung Research
Volume39
Issue number3
DOIs
StatePublished - Apr 1 2013

Keywords

  • Bronchiolitis obliterans syndrome
  • Bronchoalveolar lavage
  • Cell isolation
  • Chronic rejection
  • Fibroblasts
  • Low frequency cells
  • Mesenchymal transition

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