Isolation of cell lines from embryos of the cockroach, Blattella germanica

T. J. Kurtti, Marion A. Brooks

Research output: Contribution to journalArticlepeer-review

26 Scopus citations

Abstract

Cell lines were isolated from three stages of embryos of Blattella germanica dissociated with trypsin. The lines have been subcultured 50 to 134 times in 3 years. Line UM-BGE-1 was isolated from germ band embryos at stages of segmentation and limb-bud formation (5 days old). Line UM-BGE-2 was derived from embryos at dorsal closure (7 days old). Line UM-BGE-4 arose from embryos in the germ band and dorsal closure stages (5 and 7 days old); these cells colonize as hollow spheres or vesicles. Line UM-BGE-5, isolated during organogenesis (10 days old), developed into two distinct sublines. Subline α is composed of round cells that do not attach to the flask. Subline β grows as an attached monolayer; the cells can be removed with a saline solution containing 20mM disodium dihydrogen Versenate®. Most of the cells of these lines have the diploid chromosome number (23 or 24) excepting line UM-BGE-1 in which the tetraploid number predominates.

Original languageEnglish (US)
Pages (from-to)11-17
Number of pages7
JournalIn Vitro
Volume13
Issue number1
DOIs
StatePublished - Jan 1 1977

Keywords

  • Blattella germanica
  • diploid chromosome number
  • embryo
  • insect
  • tetraploid chromosome number

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