Isolation of cell death-associated cDNAs from involuting mouse mammary epithelium

Feng Li, Wolfgang Bielke, Guo Ke, Anne Catherine Andres, Rolf Jaggi, Susanne Saurer, Robert R. Friis, Heiner Niemann, Lynne Bemis, F. Jon Geske, Robert Strange

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

Although apoptosis is important in determining cell fate and maintaining tissue homeostasis, the initiation and control of apoptotic cell death in epithelium is not well understood. Postlactational involution of the mammary gland provides both an important developmental process and a normal physiological setting for studying apoptosis of epithelium. We used a differential screening strategy, based on previous studies correlating morphology with gene expression and nucleic acid integrity during mammary gland involution, to isolate genes involved in the regulation and execution of apoptotic cell death in regressing mammary epithelium. This screening strategy yielded a large number of genes the expression of which is significantly altered during mammary gland involution.These include genes associated with cell death processes, tissue remodelling and mesenchymal differentiation. In addition, a number of novel genes have been isolated. We have used Northern analysis and in sit u hybridisation to study the expression of a selection of these putative death-associated genes during post-lactational mouse mammary gland involution.

Original languageEnglish (US)
Pages (from-to)113-122
Number of pages10
JournalCell Death and Differentiation
Volume2
Issue number2
StatePublished - 1995

Keywords

  • Apoptosis
  • Mammary gland involution
  • Programmed cell death
  • Tissue remodelling

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