Isolation of avian pneumovirus from an outbreak of respiratory illness in Minnesota turkeys

Sagar M Goyal, Shu Ju Chiang, Arshud M. Dar, Kakambi V Nagaraja, Daniel P. Shaw, David A Halvorson, Vivek Kapur

Research output: Contribution to journalArticlepeer-review

60 Scopus citations

Abstract

Antibodies to avian pneumovirus (APV) were first detected in Minnesota turkeys in 1997. Virus isolation was attempted on 32 samples (28 tracheal swabs, 4 pools of trachea and turbinates) that were positive for APV by reverse transcriptase polymerase chain reaction (RT-PCR). The cell cultures used were chicken embryo fibroblast (CEF), Vero cells, and QT-35 cells. Five virus isolates were obtained from these samples, and the identity of the isolates was confirmed by RT-PCR. Four isolates were obtained by inoculation of CEF cells, and 1 isolate was obtained in QT-35 cells after 3-7 blind passages in cell cultures. Vero cells did not yield any isolate on primary isolation; however, all 5 isolates could be adapted to grow in Vero cells following primary isolation in CEF or QT-35 cells. This is the first report of isolation of APV in Minnesota and also the first report of primary isolation of APV in QT-35 cells.

Original languageEnglish (US)
Pages (from-to)166-168
Number of pages3
JournalJournal of Veterinary Diagnostic Investigation
Volume12
Issue number2
DOIs
StatePublished - Mar 2000

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