Isolation of Anaplasma phagocytophilum strain Ap-Variant 1 in a tick-derived cell line

Robert F. Massung; Michael L. Levin; Ulrike G. Munderloh; David J. Silverman; Meghan J. Lynch; Timothy J. Kurtti

doi:10.1196/annals.1374.105

Isolation of Anaplasma phagocytophilum strain Ap-Variant 1 in a tick-derived cell line

Robert F. Massung
, Michael L. Levin
, Ulrike G. Munderloh
, David J. Silverman
, Meghan J. Lynch
, Timothy J. Kurtti

Entomology

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

8 Scopus citations

Abstract

Ten isolates of the Ap-Variant 1 strain of Anaplasma phagocytophilum were made in the Ixodes scapularis (I. scapularis)-derived cell line, ISE6. Two isolates were obtained from laboratory-infected goats and eight isolates were obtained from field-collected I. scapularis ticks. Each isolate showed 16S rRNA sequences identical to those as previously described for the Ap-Variant 1 strain. These are the first tissue culture isolates of the Ap-Variant 1 strain and will allow for further characterization of the biological and antigenic properties of this strain.

Original language	English (US)
Title of host publication	Century of Rickettsiology; Emerging, Reemerging Rickettsioses, Molecular Diagnostics. amd Emerging Veterinary Rickettsioses
Publisher	Blackwell Publishing Inc
Pages	541-544
Number of pages	4
ISBN (Print)	1573316393, 9781573316392
DOIs	https://doi.org/10.1196/annals.1374.105
State	Published - Oct 2006

Publication series

Name	Annals of the New York Academy of Sciences
Volume	1078
ISSN (Print)	0077-8923
ISSN (Electronic)	1749-6632

Keywords

Anaplasma phagocytophilum
Ap-Variant 1
Human granulocytic anaplasmosis
Ixodes scapularis

Publisher link

10.1196/annals.1374.105

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Massung, R. F., Levin, M. L., Munderloh, U. G., Silverman, D. J., Lynch, M. J., & Kurtti, T. J. (2006). Isolation of Anaplasma phagocytophilum strain Ap-Variant 1 in a tick-derived cell line. In Century of Rickettsiology; Emerging, Reemerging Rickettsioses, Molecular Diagnostics. amd Emerging Veterinary Rickettsioses (pp. 541-544). (Annals of the New York Academy of Sciences; Vol. 1078). Blackwell Publishing Inc. https://doi.org/10.1196/annals.1374.105

Isolation of Anaplasma phagocytophilum strain Ap-Variant 1 in a tick-derived cell line. / Massung, Robert F.; Levin, Michael L.; Munderloh, Ulrike G. et al.
Century of Rickettsiology; Emerging, Reemerging Rickettsioses, Molecular Diagnostics. amd Emerging Veterinary Rickettsioses. Blackwell Publishing Inc, 2006. p. 541-544 (Annals of the New York Academy of Sciences; Vol. 1078).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Massung, RF, Levin, ML, Munderloh, UG, Silverman, DJ, Lynch, MJ & Kurtti, TJ 2006, Isolation of Anaplasma phagocytophilum strain Ap-Variant 1 in a tick-derived cell line. in Century of Rickettsiology; Emerging, Reemerging Rickettsioses, Molecular Diagnostics. amd Emerging Veterinary Rickettsioses. Annals of the New York Academy of Sciences, vol. 1078, Blackwell Publishing Inc, pp. 541-544. https://doi.org/10.1196/annals.1374.105

Massung RF, Levin ML, Munderloh UG, Silverman DJ, Lynch MJ, Kurtti TJ. Isolation of Anaplasma phagocytophilum strain Ap-Variant 1 in a tick-derived cell line. In Century of Rickettsiology; Emerging, Reemerging Rickettsioses, Molecular Diagnostics. amd Emerging Veterinary Rickettsioses. Blackwell Publishing Inc. 2006. p. 541-544. (Annals of the New York Academy of Sciences). doi: 10.1196/annals.1374.105

Massung, Robert F. ; Levin, Michael L. ; Munderloh, Ulrike G. et al. / Isolation of Anaplasma phagocytophilum strain Ap-Variant 1 in a tick-derived cell line. Century of Rickettsiology; Emerging, Reemerging Rickettsioses, Molecular Diagnostics. amd Emerging Veterinary Rickettsioses. Blackwell Publishing Inc, 2006. pp. 541-544 (Annals of the New York Academy of Sciences).

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title = "Isolation of Anaplasma phagocytophilum strain Ap-Variant 1 in a tick-derived cell line",

abstract = "Ten isolates of the Ap-Variant 1 strain of Anaplasma phagocytophilum were made in the Ixodes scapularis (I. scapularis)-derived cell line, ISE6. Two isolates were obtained from laboratory-infected goats and eight isolates were obtained from field-collected I. scapularis ticks. Each isolate showed 16S rRNA sequences identical to those as previously described for the Ap-Variant 1 strain. These are the first tissue culture isolates of the Ap-Variant 1 strain and will allow for further characterization of the biological and antigenic properties of this strain.",

keywords = "Anaplasma phagocytophilum, Ap-Variant 1, Human granulocytic anaplasmosis, Ixodes scapularis",

author = "Massung, \{Robert F.\} and Levin, \{Michael L.\} and Munderloh, \{Ulrike G.\} and Silverman, \{David J.\} and Lynch, \{Meghan J.\} and Kurtti, \{Timothy J.\}",

year = "2006",

month = oct,

doi = "10.1196/annals.1374.105",

language = "English (US)",

isbn = "1573316393",

series = "Annals of the New York Academy of Sciences",

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AU - Massung, Robert F.

AU - Levin, Michael L.

AU - Munderloh, Ulrike G.

AU - Silverman, David J.

AU - Lynch, Meghan J.

AU - Kurtti, Timothy J.

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N2 - Ten isolates of the Ap-Variant 1 strain of Anaplasma phagocytophilum were made in the Ixodes scapularis (I. scapularis)-derived cell line, ISE6. Two isolates were obtained from laboratory-infected goats and eight isolates were obtained from field-collected I. scapularis ticks. Each isolate showed 16S rRNA sequences identical to those as previously described for the Ap-Variant 1 strain. These are the first tissue culture isolates of the Ap-Variant 1 strain and will allow for further characterization of the biological and antigenic properties of this strain.

AB - Ten isolates of the Ap-Variant 1 strain of Anaplasma phagocytophilum were made in the Ixodes scapularis (I. scapularis)-derived cell line, ISE6. Two isolates were obtained from laboratory-infected goats and eight isolates were obtained from field-collected I. scapularis ticks. Each isolate showed 16S rRNA sequences identical to those as previously described for the Ap-Variant 1 strain. These are the first tissue culture isolates of the Ap-Variant 1 strain and will allow for further characterization of the biological and antigenic properties of this strain.

KW - Anaplasma phagocytophilum

KW - Ap-Variant 1

KW - Human granulocytic anaplasmosis

KW - Ixodes scapularis

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DO - 10.1196/annals.1374.105

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T3 - Annals of the New York Academy of Sciences

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BT - Century of Rickettsiology; Emerging, Reemerging Rickettsioses, Molecular Diagnostics. amd Emerging Veterinary Rickettsioses

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Isolation of Anaplasma phagocytophilum strain Ap-Variant 1 in a tick-derived cell line

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