Isolation of Anaplasma phagocytophilum strain Ap-Variant 1 in a tick-derived cell line

Robert F. Massung, Michael L. Levin, Ulrike G. Munderloh, David J. Silverman, Meghan J. Lynch, Timothy J. Kurtti

Research output: Chapter in Book/Report/Conference proceedingConference contribution

8 Scopus citations

Abstract

Ten isolates of the Ap-Variant 1 strain of Anaplasma phagocytophilum were made in the Ixodes scapularis (I. scapularis)-derived cell line, ISE6. Two isolates were obtained from laboratory-infected goats and eight isolates were obtained from field-collected I. scapularis ticks. Each isolate showed 16S rRNA sequences identical to those as previously described for the Ap-Variant 1 strain. These are the first tissue culture isolates of the Ap-Variant 1 strain and will allow for further characterization of the biological and antigenic properties of this strain.

Original languageEnglish (US)
Title of host publicationCentury of Rickettsiology; Emerging, Reemerging Rickettsioses, Molecular Diagnostics. amd Emerging Veterinary Rickettsioses
PublisherBlackwell Publishing Inc
Pages541-544
Number of pages4
ISBN (Print)1573316393, 9781573316392
DOIs
StatePublished - Oct 2006

Publication series

NameAnnals of the New York Academy of Sciences
Volume1078
ISSN (Print)0077-8923
ISSN (Electronic)1749-6632

Keywords

  • Anaplasma phagocytophilum
  • Ap-Variant 1
  • Human granulocytic anaplasmosis
  • Ixodes scapularis

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    Massung, R. F., Levin, M. L., Munderloh, U. G., Silverman, D. J., Lynch, M. J., & Kurtti, T. J. (2006). Isolation of Anaplasma phagocytophilum strain Ap-Variant 1 in a tick-derived cell line. In Century of Rickettsiology; Emerging, Reemerging Rickettsioses, Molecular Diagnostics. amd Emerging Veterinary Rickettsioses (pp. 541-544). (Annals of the New York Academy of Sciences; Vol. 1078). Blackwell Publishing Inc. https://doi.org/10.1196/annals.1374.105