Isolation of a novel cDNA encoding a putative membrane receptor with high homology to the cloned μ, δ, and κ opioid receptors

Marilee J. Wick; Sharon R. Minnerath; Xiaoqin Lin; Robert Elde; Ping Y. Law; Horace H Loh

doi:10.1016/0169-328X(94)90181-3

Isolation of a novel cDNA encoding a putative membrane receptor with high homology to the cloned μ, δ, and κ opioid receptors

Marilee J. Wick, Sharon R. Minnerath, Xiaoqin Lin, Robert Elde, Ping Y. Law, Horace H Loh

Research output: Contribution to journal › Article › peer-review

230 Scopus citations

Abstract

A rat brain cDNA library was screened for clones homologous to the recently cloned mouse δ-opioid receptor (DOR-1). Among the clones isolated was Hyp 8-1, a clone with a unique nucleotide sequence capable of encoding a putative protein which is 57-58% identical to the amino acid sequences of the cloned δ, μ and κ opioid receptors, indicating a close relationship of Hyp 8-1 with the opioid receptor family. Several cDNAs representing possible splice variants of Hyp 8-1 were also isolated. Binding studies of COS-7 cells transfected with clone Hyp 8-1 failed to demonstrate specific binding with several ³H-opioid ligands. In situ hybridization studies indicate that the mRNA for Hyp 8-1 is distributed discretely throughout the rat brain, in an overall pattern which is different from that of several other G-protein-coupled seven transmembrane receptors. Thus, it is likely that the Hyp 8-1 cDNA encodes a novel peptide receptor.

Original language	English (US)
Pages (from-to)	37-44
Number of pages	8
Journal	Molecular Brain Research
Volume	27
Issue number	1
DOIs	https://doi.org/10.1016/0169-328X(94)90181-3
State	Published - Nov 1994

Bibliographical note

Funding Information:
We wish to thank Dr. Andrew Smith for help in sequence analysis. This work was suppported by NIH Grants DA 00564, DA 01583, DA 05695, K05-DA-70554 and F&A Stark Fund of the Minnesota Medical Foundation (H.H.L.) and DA 06299 and program project grants (R.E.)

Keywords

In situ hybridization
Opioid receptor
Rat brain
Transient expression
Transmembrane protein
cDNA cloning

Publisher link

10.1016/0169-328X(94)90181-3

Find It

Find It at U of M Libraries

Cite this

@article{07a2ea373f2e489ca3b80968c68eb82a,

title = "Isolation of a novel cDNA encoding a putative membrane receptor with high homology to the cloned μ, δ, and κ opioid receptors",

abstract = "A rat brain cDNA library was screened for clones homologous to the recently cloned mouse δ-opioid receptor (DOR-1). Among the clones isolated was Hyp 8-1, a clone with a unique nucleotide sequence capable of encoding a putative protein which is 57-58% identical to the amino acid sequences of the cloned δ, μ and κ opioid receptors, indicating a close relationship of Hyp 8-1 with the opioid receptor family. Several cDNAs representing possible splice variants of Hyp 8-1 were also isolated. Binding studies of COS-7 cells transfected with clone Hyp 8-1 failed to demonstrate specific binding with several 3H-opioid ligands. In situ hybridization studies indicate that the mRNA for Hyp 8-1 is distributed discretely throughout the rat brain, in an overall pattern which is different from that of several other G-protein-coupled seven transmembrane receptors. Thus, it is likely that the Hyp 8-1 cDNA encodes a novel peptide receptor.",

keywords = "In situ hybridization, Opioid receptor, Rat brain, Transient expression, Transmembrane protein, cDNA cloning",

author = "Wick, {Marilee J.} and Minnerath, {Sharon R.} and Xiaoqin Lin and Robert Elde and Law, {Ping Y.} and Loh, {Horace H}",

note = "Funding Information: We wish to thank Dr. Andrew Smith for help in sequence analysis. This work was suppported by NIH Grants DA 00564, DA 01583, DA 05695, K05-DA-70554 and F&A Stark Fund of the Minnesota Medical Foundation (H.H.L.) and DA 06299 and program project grants (R.E.)",

year = "1994",

month = nov,

doi = "10.1016/0169-328X(94)90181-3",

language = "English (US)",

volume = "27",

pages = "37--44",

journal = "Brain Research",

issn = "0006-8993",

publisher = "Elsevier",

number = "1",

}

TY - JOUR

T1 - Isolation of a novel cDNA encoding a putative membrane receptor with high homology to the cloned μ, δ, and κ opioid receptors

AU - Wick, Marilee J.

AU - Minnerath, Sharon R.

AU - Lin, Xiaoqin

AU - Elde, Robert

AU - Law, Ping Y.

AU - Loh, Horace H

N1 - Funding Information: We wish to thank Dr. Andrew Smith for help in sequence analysis. This work was suppported by NIH Grants DA 00564, DA 01583, DA 05695, K05-DA-70554 and F&A Stark Fund of the Minnesota Medical Foundation (H.H.L.) and DA 06299 and program project grants (R.E.)

PY - 1994/11

Y1 - 1994/11

N2 - A rat brain cDNA library was screened for clones homologous to the recently cloned mouse δ-opioid receptor (DOR-1). Among the clones isolated was Hyp 8-1, a clone with a unique nucleotide sequence capable of encoding a putative protein which is 57-58% identical to the amino acid sequences of the cloned δ, μ and κ opioid receptors, indicating a close relationship of Hyp 8-1 with the opioid receptor family. Several cDNAs representing possible splice variants of Hyp 8-1 were also isolated. Binding studies of COS-7 cells transfected with clone Hyp 8-1 failed to demonstrate specific binding with several 3H-opioid ligands. In situ hybridization studies indicate that the mRNA for Hyp 8-1 is distributed discretely throughout the rat brain, in an overall pattern which is different from that of several other G-protein-coupled seven transmembrane receptors. Thus, it is likely that the Hyp 8-1 cDNA encodes a novel peptide receptor.

AB - A rat brain cDNA library was screened for clones homologous to the recently cloned mouse δ-opioid receptor (DOR-1). Among the clones isolated was Hyp 8-1, a clone with a unique nucleotide sequence capable of encoding a putative protein which is 57-58% identical to the amino acid sequences of the cloned δ, μ and κ opioid receptors, indicating a close relationship of Hyp 8-1 with the opioid receptor family. Several cDNAs representing possible splice variants of Hyp 8-1 were also isolated. Binding studies of COS-7 cells transfected with clone Hyp 8-1 failed to demonstrate specific binding with several 3H-opioid ligands. In situ hybridization studies indicate that the mRNA for Hyp 8-1 is distributed discretely throughout the rat brain, in an overall pattern which is different from that of several other G-protein-coupled seven transmembrane receptors. Thus, it is likely that the Hyp 8-1 cDNA encodes a novel peptide receptor.

KW - In situ hybridization

KW - Opioid receptor

KW - Rat brain

KW - Transient expression

KW - Transmembrane protein

KW - cDNA cloning

UR - http://www.scopus.com/inward/record.url?scp=0028072363&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028072363&partnerID=8YFLogxK

U2 - 10.1016/0169-328X(94)90181-3

DO - 10.1016/0169-328X(94)90181-3

M3 - Article

C2 - 7877452

AN - SCOPUS:0028072363

VL - 27

SP - 37

EP - 44

JO - Brain Research

JF - Brain Research

SN - 0006-8993

IS - 1

ER -

Isolation of a novel cDNA encoding a putative membrane receptor with high homology to the cloned μ, δ, and κ opioid receptors

Abstract

Bibliographical note

Keywords

Publisher link

Find It

Other files and links

Fingerprint

Cite this