Abstract
A rat brain cDNA library was screened for clones homologous to the recently cloned mouse δ-opioid receptor (DOR-1). Among the clones isolated was Hyp 8-1, a clone with a unique nucleotide sequence capable of encoding a putative protein which is 57-58% identical to the amino acid sequences of the cloned δ, μ and κ opioid receptors, indicating a close relationship of Hyp 8-1 with the opioid receptor family. Several cDNAs representing possible splice variants of Hyp 8-1 were also isolated. Binding studies of COS-7 cells transfected with clone Hyp 8-1 failed to demonstrate specific binding with several 3H-opioid ligands. In situ hybridization studies indicate that the mRNA for Hyp 8-1 is distributed discretely throughout the rat brain, in an overall pattern which is different from that of several other G-protein-coupled seven transmembrane receptors. Thus, it is likely that the Hyp 8-1 cDNA encodes a novel peptide receptor.
Original language | English (US) |
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Pages (from-to) | 37-44 |
Number of pages | 8 |
Journal | Molecular Brain Research |
Volume | 27 |
Issue number | 1 |
DOIs | |
State | Published - Nov 1994 |
Keywords
- In situ hybridization
- Opioid receptor
- Rat brain
- Transient expression
- Transmembrane protein
- cDNA cloning