Isolation of a novel cDNA encoding a putative membrane receptor with high homology to the cloned μ, δ, and κ opioid receptors

Marilee J. Wick, Sharon R. Minnerath, Xiaoqin Lin, Robert Elde, Ping Y. Law, Horace H Loh

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226 Scopus citations

Abstract

A rat brain cDNA library was screened for clones homologous to the recently cloned mouse δ-opioid receptor (DOR-1). Among the clones isolated was Hyp 8-1, a clone with a unique nucleotide sequence capable of encoding a putative protein which is 57-58% identical to the amino acid sequences of the cloned δ, μ and κ opioid receptors, indicating a close relationship of Hyp 8-1 with the opioid receptor family. Several cDNAs representing possible splice variants of Hyp 8-1 were also isolated. Binding studies of COS-7 cells transfected with clone Hyp 8-1 failed to demonstrate specific binding with several 3H-opioid ligands. In situ hybridization studies indicate that the mRNA for Hyp 8-1 is distributed discretely throughout the rat brain, in an overall pattern which is different from that of several other G-protein-coupled seven transmembrane receptors. Thus, it is likely that the Hyp 8-1 cDNA encodes a novel peptide receptor.

Original languageEnglish (US)
Pages (from-to)37-44
Number of pages8
JournalMolecular Brain Research
Volume27
Issue number1
DOIs
StatePublished - Nov 1994

Keywords

  • In situ hybridization
  • Opioid receptor
  • Rat brain
  • Transient expression
  • Transmembrane protein
  • cDNA cloning

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