Isolation of a novel cDNA encoding a putative membrane receptor with high homology to the cloned μ, δ, and κ opioid receptors

Marilee J. Wick; Sharon R. Minnerath; Xiaoqin Lin; Robert Elde; Ping Y. Law; Horace H Loh

doi:10.1016/0169-328X(94)90181-3

Isolation of a novel cDNA encoding a putative membrane receptor with high homology to the cloned μ, δ, and κ opioid receptors

Marilee J. Wick, Sharon R. Minnerath, Xiaoqin Lin, Robert Elde, Ping Y. Law, Horace H Loh

Research output: Contribution to journal › Article › peer-review

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Abstract

A rat brain cDNA library was screened for clones homologous to the recently cloned mouse δ-opioid receptor (DOR-1). Among the clones isolated was Hyp 8-1, a clone with a unique nucleotide sequence capable of encoding a putative protein which is 57-58% identical to the amino acid sequences of the cloned δ, μ and κ opioid receptors, indicating a close relationship of Hyp 8-1 with the opioid receptor family. Several cDNAs representing possible splice variants of Hyp 8-1 were also isolated. Binding studies of COS-7 cells transfected with clone Hyp 8-1 failed to demonstrate specific binding with several ³H-opioid ligands. In situ hybridization studies indicate that the mRNA for Hyp 8-1 is distributed discretely throughout the rat brain, in an overall pattern which is different from that of several other G-protein-coupled seven transmembrane receptors. Thus, it is likely that the Hyp 8-1 cDNA encodes a novel peptide receptor.

Original language	English (US)
Pages (from-to)	37-44
Number of pages	8
Journal	Molecular Brain Research
Volume	27
Issue number	1
DOIs	https://doi.org/10.1016/0169-328X(94)90181-3
State	Published - Nov 1994

Bibliographical note

Funding Information:
We wish to thank Dr. Andrew Smith for help in sequence analysis. This work was suppported by NIH Grants DA 00564, DA 01583, DA 05695, K05-DA-70554 and F&A Stark Fund of the Minnesota Medical Foundation (H.H.L.) and DA 06299 and program project grants (R.E.)

Keywords

In situ hybridization
Opioid receptor
Rat brain
Transient expression
Transmembrane protein
cDNA cloning

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title = "Isolation of a novel cDNA encoding a putative membrane receptor with high homology to the cloned μ, δ, and κ opioid receptors",

abstract = "A rat brain cDNA library was screened for clones homologous to the recently cloned mouse δ-opioid receptor (DOR-1). Among the clones isolated was Hyp 8-1, a clone with a unique nucleotide sequence capable of encoding a putative protein which is 57-58% identical to the amino acid sequences of the cloned δ, μ and κ opioid receptors, indicating a close relationship of Hyp 8-1 with the opioid receptor family. Several cDNAs representing possible splice variants of Hyp 8-1 were also isolated. Binding studies of COS-7 cells transfected with clone Hyp 8-1 failed to demonstrate specific binding with several 3H-opioid ligands. In situ hybridization studies indicate that the mRNA for Hyp 8-1 is distributed discretely throughout the rat brain, in an overall pattern which is different from that of several other G-protein-coupled seven transmembrane receptors. Thus, it is likely that the Hyp 8-1 cDNA encodes a novel peptide receptor.",

keywords = "In situ hybridization, Opioid receptor, Rat brain, Transient expression, Transmembrane protein, cDNA cloning",

author = "Wick, {Marilee J.} and Minnerath, {Sharon R.} and Xiaoqin Lin and Robert Elde and Law, {Ping Y.} and Loh, {Horace H}",

note = "Funding Information: We wish to thank Dr. Andrew Smith for help in sequence analysis. This work was suppported by NIH Grants DA 00564, DA 01583, DA 05695, K05-DA-70554 and F&A Stark Fund of the Minnesota Medical Foundation (H.H.L.) and DA 06299 and program project grants (R.E.)",

year = "1994",

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doi = "10.1016/0169-328X(94)90181-3",

language = "English (US)",

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AU - Wick, Marilee J.

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AU - Lin, Xiaoqin

AU - Elde, Robert

AU - Law, Ping Y.

AU - Loh, Horace H

N1 - Funding Information: We wish to thank Dr. Andrew Smith for help in sequence analysis. This work was suppported by NIH Grants DA 00564, DA 01583, DA 05695, K05-DA-70554 and F&A Stark Fund of the Minnesota Medical Foundation (H.H.L.) and DA 06299 and program project grants (R.E.)

PY - 1994/11

Y1 - 1994/11

N2 - A rat brain cDNA library was screened for clones homologous to the recently cloned mouse δ-opioid receptor (DOR-1). Among the clones isolated was Hyp 8-1, a clone with a unique nucleotide sequence capable of encoding a putative protein which is 57-58% identical to the amino acid sequences of the cloned δ, μ and κ opioid receptors, indicating a close relationship of Hyp 8-1 with the opioid receptor family. Several cDNAs representing possible splice variants of Hyp 8-1 were also isolated. Binding studies of COS-7 cells transfected with clone Hyp 8-1 failed to demonstrate specific binding with several 3H-opioid ligands. In situ hybridization studies indicate that the mRNA for Hyp 8-1 is distributed discretely throughout the rat brain, in an overall pattern which is different from that of several other G-protein-coupled seven transmembrane receptors. Thus, it is likely that the Hyp 8-1 cDNA encodes a novel peptide receptor.

AB - A rat brain cDNA library was screened for clones homologous to the recently cloned mouse δ-opioid receptor (DOR-1). Among the clones isolated was Hyp 8-1, a clone with a unique nucleotide sequence capable of encoding a putative protein which is 57-58% identical to the amino acid sequences of the cloned δ, μ and κ opioid receptors, indicating a close relationship of Hyp 8-1 with the opioid receptor family. Several cDNAs representing possible splice variants of Hyp 8-1 were also isolated. Binding studies of COS-7 cells transfected with clone Hyp 8-1 failed to demonstrate specific binding with several 3H-opioid ligands. In situ hybridization studies indicate that the mRNA for Hyp 8-1 is distributed discretely throughout the rat brain, in an overall pattern which is different from that of several other G-protein-coupled seven transmembrane receptors. Thus, it is likely that the Hyp 8-1 cDNA encodes a novel peptide receptor.

KW - In situ hybridization

KW - Opioid receptor

KW - Rat brain

KW - Transient expression

KW - Transmembrane protein

KW - cDNA cloning

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Isolation of a novel cDNA encoding a putative membrane receptor with high homology to the cloned μ, δ, and κ opioid receptors

Abstract

Bibliographical note

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