Abstract
A mouse genomic library in λCharon 4A was screened for putative ribosomal protein genes using a fragment of the gene encoding Drosophila ribosomal protein 49 as a hybridization probe under nonstringent hybridization conditions. A recombinant phage was selected and its restriction enzyme map determined. The major species of mouse poly(A)+ mRNA homologous to the putative gene is about 740 nucleotides long.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 155-161 |
| Number of pages | 7 |
| Journal | Gene |
| Volume | 34 |
| Issue number | 2-3 |
| DOIs | |
| State | Published - 1985 |
Bibliographical note
Funding Information:We are indebtedt o Drs. M. Davis for providing us with the mouseg enomicl ibraryin lCharon 4A, M. Rosbashfo r supplyingth eD rosophila r-protein4 9 genec lone, HR0.6, and T. Brandt for mousec ell poly(A)‘mRNA. This researchw as supportedb y grant PCM 8010975f rom the National Science Foundation,a nd funds from an AmericanC ancer SocietyI nstitutionaRl esearchG rant to the University of Minnesota.
Keywords
- Recombinant DNA
- evolutionary conservation
- heterologous probe
- nonstringent hybridization
- λCharon phage vectors
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