Isolation of a mouse DNA fragment with homology to a Drosophila ribosomal protein gene

Pamela K. Mulligan, Perry B. Hackett

Research output: Contribution to journalArticlepeer-review

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Abstract

A mouse genomic library in λCharon 4A was screened for putative ribosomal protein genes using a fragment of the gene encoding Drosophila ribosomal protein 49 as a hybridization probe under nonstringent hybridization conditions. A recombinant phage was selected and its restriction enzyme map determined. The major species of mouse poly(A)+ mRNA homologous to the putative gene is about 740 nucleotides long.

Original languageEnglish (US)
Pages (from-to)155-161
Number of pages7
JournalGene
Volume34
Issue number2-3
DOIs
StatePublished - 1985

Bibliographical note

Funding Information:
We are indebtedt o Drs. M. Davis for providing us with the mouseg enomicl ibraryin lCharon 4A, M. Rosbashfo r supplyingth eD rosophila r-protein4 9 genec lone, HR0.6, and T. Brandt for mousec ell poly(A)‘mRNA. This researchw as supportedb y grant PCM 8010975f rom the National Science Foundation,a nd funds from an AmericanC ancer SocietyI nstitutionaRl esearchG rant to the University of Minnesota.

Keywords

  • Recombinant DNA
  • evolutionary conservation
  • heterologous probe
  • nonstringent hybridization
  • λCharon phage vectors

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